厚朴酚是一种从厚朴中提取的强效抗氧化剂，可减轻高胆固醇饮食家兔主动脉球囊损伤后的内膜增厚和单核细胞趋化蛋白-1（MCP-1）表达。

Magnolol, a potent antioxidant from Magnolia officinalis, attenuates intimal thickening and MCP-1 expression after balloon injury of the aorta in cholesterol-fed rabbits.

作者信息

Chen Y L, Lin K F, Shiao M S, Chen Y T, Hong C Y, Lin S J

机构信息

Institute of Anatomy and Cell Biology, National Yang-Ming University, Taipei, Taiwan.

出版信息

Basic Res Cardiol. 2001 Jul;96(4):353-63. doi: 10.1007/s003950170043.

DOI:10.1007/s003950170043

PMID:11518191

Abstract

BACKGROUND

Restenosis is a common complication after balloon angioplasty. A number of cytokines, chemotactic factors and growth factors may be involved. Several antioxidants have been shown to inhibit intimal thickening after balloon injury in hyperlipidemic animals.

OBJECTIVES

The effects of magnolol on the expression of monocyte chemotactic protein-1 (MCP-1) and intimal response in balloon injured aorta of cholesterol-fed rabbits were investigated.

METHODS

Male New Zealand white rabbits were fed a 2% high cholesterol (HC) diet together with daily intramuscular injection of either 1 microg/kg B.W. of magnolol (HC-M, n = 10) or vehicle (propylene glycol) as a control (HC-C, n = 10) for a total of 6 weeks. Another 10 rabbits fed a regular diet also served as a control (C) group. A balloon denudation of abdominal aorta was performed in each group at the end of the third week. The aortas were harvested at the end of 6 weeks.

RESULTS

Magnolol treatment significantly inhibited Cu2+-induced LDL oxidation in cholesterol-fed rabbits and reduced atheroma formation [atheroma area ratio: 0.10 +/- 0.03 (HC-M) versus 0.33 +/- 0.07 (HC-C), p < 0.05] in thoracic aortas without lowering serum cholesterol. The intimal response was significantly attenuated in the HC-M rabbits when compared to those of the HC-C group [intimal thickness: 88.95 +/- 14.91 microm (HC-M) versus 198.02 +/- 20.35 microm (HC-C), p < 0.05; intimal area: 278.21 +/- 43.16 x 10(3) microm2 (HC-M) versus 642.70 +/- 65.01 x 10(3) microm2 (HC-C), p < 0.05]. The MCP-1 mRNA and protein expression were reduced in the HC-M group compared to the HC-C and C groups.

CONCLUSION

The inhibitory effects on intimal hyperplasia and MCP-1 expression might be attributed to the antioxidant capacity of magnolol instead of lowering serum cholesterol. Magnolol may offer some protection against postangioplasty restenosis.

摘要

背景

再狭窄是球囊血管成形术后常见的并发症。多种细胞因子、趋化因子和生长因子可能与之相关。已有研究表明，多种抗氧化剂可抑制高脂血症动物球囊损伤后的内膜增厚。

目的

研究厚朴酚对高胆固醇喂养兔球囊损伤主动脉中单核细胞趋化蛋白-1（MCP-1）表达及内膜反应的影响。

方法

雄性新西兰白兔喂食2%高胆固醇（HC）饮食，同时每日肌肉注射1μg/kg体重的厚朴酚（HC-M组，n = 10）或溶剂（丙二醇）作为对照（HC-C组，n = 10），共6周。另外10只喂食常规饮食的兔子作为对照（C）组。在第三周结束时，对每组兔子进行腹主动脉球囊剥脱术。6周结束时收获主动脉。

结果

厚朴酚治疗可显著抑制高胆固醇喂养兔Cu2+诱导的低密度脂蛋白氧化，并减少胸主动脉粥样硬化形成[粥样硬化面积比：0.10±0.03（HC-M）对0.33±0.07（HC-C），p < 0.05]，且不降低血清胆固醇。与HC-C组相比，HC-M组兔子的内膜反应明显减弱[内膜厚度：88.95±14.91μm（HC-M）对198.02±20.35μm（HC-C），p < 0.05；内膜面积：278.21±43.16×10(3)μm2（HC-M）对642.70±65.01×10(3)μm2（HC-C），p < 0.05]。与HC-C组和C组相比，HC-M组中MCP-1 mRNA和蛋白表达降低。