Clark T L, Meinke L J, Foster J E
Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, USA.
Insect Mol Biol. 2001 Aug;10(4):303-14. doi: 10.1046/j.0962-1075.2001.00269.x.
The phylogenetic relationships of thirteen Diabrotica (representing virgifera and fucata species groups) and two outgroup Acalymma beetle species (Coleoptera: Chrysomelidae) were inferred from the phylogenetic analysis of a combined data set of 1323 bp of mitochondrial DNA (mtDNA) cytochrome oxidase subunit 1 (COI) and the entire second internal transcribed spacer region (ITS-2) of nuclear ribosomal DNA of 362 characters. Species investigated were D. adelpha, D. balteata, D. barberi, D. cristata, D. lemniscata, D. longicornis, D. porracea, D. speciosa, D. undecimpunctata howardi, D. u. undecimpunctata, D. virgifera virgifera, D. v. zeae, D. viridula, and outgroup A. blandulum and A. vittatum. Maximum parsimony (MP), minimum evolution (ME), and maximum likelihood (ML) analyses of combined COI and ITS-2 sequences clearly place species into their traditional morphological species groups with MP and ME analyses resulting in identical topologies. Results generally confer with a prior work based on allozyme data, but within the virgifera species group, D. barberi and D. longicornis strongly resolve as sister taxa as well as monophyletic with the neotropical species, D. viridula, D. cristata and D. lemniscata also resolve as sister taxa. Both relationships are not in congruence with the prior allozyme-based hypothesis. Within the fucata species group, D. speciosa and D. balteata resolve as sister taxa. Results also strongly supported the D. virgifera and D. undecimpunctata subspecies complexes. Our proposed phylogeny provides some insight into current hypotheses regarding distribution status and evolution of various life history traits for Diabrotica.
通过对包含1323个碱基对的线粒体DNA（mtDNA）细胞色素氧化酶亚基1（COI）以及362个碱基对的核糖体DNA第二内部转录间隔区（ITS-2）的联合数据集进行系统发育分析，推断出了13种黄瓜甲虫（代表virgifera和fucata物种组）以及2种外群Acalymma甲虫物种（鞘翅目：叶甲科）之间的系统发育关系。所研究的物种包括D. adelpha、D. balteata、D. barberi、D. cristata、D. lemniscata、D. longicornis、D. porracea、D. speciosa、D. undecimpunctata howardi、D. u. undecimpunctata、D. virgifera virgifera、D. v. zeae、D. viridula，外群为A. blandulum和A. vittatum。对COI和ITS-2联合序列进行的最大简约法（MP）、最小进化法（ME）和最大似然法（ML）分析清楚地将各物种归入其传统形态物种组，MP和ME分析得出相同的拓扑结构。结果总体上与基于等位酶数据的先前研究一致，但在virgifera物种组内，D. barberi和D. longicornis强烈解析为姐妹类群，并且与新热带物种单系，D. viridula、D. cristata和D. lemniscata也解析为姐妹类群。这两种关系均与基于先前等位酶的假说不一致。在fucata物种组内，D. speciosa和D. balteata解析为姐妹类群。结果还强烈支持D. virgifera和D. undecimpunctata亚种复合体。我们提出的系统发育为当前关于黄瓜甲虫各种生活史特征的分布状况和进化的假说提供了一些见解。
