Seppä H E, Järvinen M
Acta Histochem. 1979;64(2):174-8. doi: 10.1016/S0065-1281(79)80069-0.
The highly sensitive PAP immunoperoxidase method was used to localize the main neutral protease of rat skin. The use of the neutral detergent, Triton X-100, in the reagent and washing solutions was observed to effectively decrease the nonspecific staining. The specific staining was localized to the mast cell granules.
采用高灵敏度的PAP免疫过氧化物酶法对大鼠皮肤的主要中性蛋白酶进行定位。观察到在试剂和洗涤液中使用中性去污剂Triton X-100可有效减少非特异性染色。特异性染色定位于肥大细胞颗粒。