Seppä H E
J Invest Dermatol. 1978 Nov;71(5):311-5. doi: 10.1111/1523-1747.ep12529791.
Specific antiserum against the purified rat skin main neutral protease was used in double layer immunofluorescent method to localize the enzyme in normal rat skin. The specific immunofluorescence was seen in dermal cells that were identified as mast cells on basis of their metachromatic granules. Enzyme histochemical staining with naphthol AS-D chloroacetate localized to the same cells that exhibited specific immunofluorescence. The granules of isolated rat mast cells also gave a specific reaction with the immunohistochemical technique. The results provide further evidence for the suggestion that rat skin main neutral protease is identical with the rat mast cell "chymase."
采用针对纯化的大鼠皮肤主要中性蛋白酶的特异性抗血清，运用双层免疫荧光法在正常大鼠皮肤中定位该酶。在真皮细胞中可见特异性免疫荧光，根据其异染颗粒鉴定这些细胞为肥大细胞。用萘酚AS-D氯乙酸酯进行的酶组织化学染色定位于呈现特异性免疫荧光的相同细胞。分离的大鼠肥大细胞的颗粒用免疫组织化学技术也产生特异性反应。这些结果为大鼠皮肤主要中性蛋白酶与大鼠肥大细胞“糜酶”相同这一观点提供了进一步的证据。
