Teruyama K, Abe M, Nakano T, Takahashi S, Yamada S, Sato Y
Department of Vascular Biology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
FEBS Lett. 2001 Aug 24;504(1-2):1-4. doi: 10.1016/s0014-5793(01)02724-7.
Transcription factor Ets-1 expressed in endothelial cells promotes angiogenesis. Here, we transiently overexpressed Ets-1 in human umbilical vein endothelial cells (HUVECs) and comprehensively searched for potential downstream targets of Ets-1 by cDNA microarray analysis. The expression of several angiogenesis-related genes including neuropilin-1 was augmented by the overexpression of Ets-1. Quantitative real-time RT-PCR and Western blotting confirmed the increase in the levels of neuropilin-1 mRNA and protein. In contrast, dominant negative ets-1 decreased the levels of neuropilin-1 mRNA and protein. These results indicate that neuropilin-1 is a downstream target of Ets-1 in HUVECs.
在内皮细胞中表达的转录因子Ets-1可促进血管生成。在此，我们在人脐静脉内皮细胞（HUVECs）中瞬时过表达Ets-1，并通过cDNA微阵列分析全面搜索Ets-1的潜在下游靶点。包括神经纤毛蛋白-1在内的几种血管生成相关基因的表达因Ets-1的过表达而增加。定量实时RT-PCR和蛋白质印迹证实了神经纤毛蛋白-1 mRNA和蛋白质水平的增加。相反，显性负性ets-1降低了神经纤毛蛋白-1 mRNA和蛋白质水平。这些结果表明，神经纤毛蛋白-1是HUVECs中Ets-1的下游靶点。
