Yano H, Wong J H, Cho M J, Buchanan B B
Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA 94720, USA.
Plant Cell Physiol. 2001 Aug;42(8):879-83. doi: 10.1093/pcp/pce119.
The mobilization of storage proteins (glutelins) in germinating rice seeds was accompanied by an ordered sequential combination of proteolysis and reduction of disulfide groups. Mobilization was followed by application of non-reducing/reducing two dimensional-PAGE after monobromobimane labeling of the sulfhydryl groups of the proteins in intact seeds.
发芽水稻种子中贮藏蛋白（谷蛋白）的动员伴随着蛋白水解作用与二硫键还原的有序顺序组合。在对完整种子中蛋白质的巯基进行单溴代联苯胺标记后，通过非还原/还原双向聚丙烯酰胺凝胶电泳对动员过程进行分析。
