Tao X, Li G, Luo S
Department of Traditional Chinese Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
J Tongji Med Univ. 2001;21(2):105-7. doi: 10.1007/BF02888068.
To investigate the effect of Jianpiyiqi prescription on the expression of heat shock proteins (HSPs) and the changes of HSPs in gastric-ulcerated rats, the rat model of chronic gastric ulcer was induced by acetic acid. The SABC immunohistochemical method was used to observe HSP70 of mucosa around the gastric ulcer. Imaging analysis was performed. Western dot blot was used to detect HSPs contents in the plasma and gastric mucosal homogenate in each group. The results showed that HSP70 contents of the mucosa around the gastric ulcer in the model group and ranitidine-treated group were increased as compared with control group (P < 0.01). Jianpiyiqi could increase the expression of HSP70 of the mucosa around the gastric ulcer further as compared with that in the model group and ranitidine-treated group (P < 0.01). The HSP70 contents in the serum and mucosa in the model group and ranitidine-treated group were increased as compared with control group (P < 0.01, P < 0.05 respectively). HSP70 of serum and mucosa in the Jianpiyiqi-treated group was higher than in the model group and ranitidine-treated group (P < 0.05, P < 0.01 respectively). It was concluded that HSP might play a role in the process of pathophysiology of gastric ulcer. Jianpiyiqi could enhance gastric ulcer-healing through the protective mechanism of HSPs.
为探讨健脾益气方对胃溃疡大鼠热休克蛋白（HSPs）表达及HSPs变化的影响，采用醋酸诱导大鼠慢性胃溃疡模型。运用SABC免疫组化法观察胃溃疡周围黏膜的HSP70，进行图像分析。采用Western dot blot法检测各组血浆及胃黏膜匀浆中HSPs含量。结果显示，模型组和雷尼替丁治疗组胃溃疡周围黏膜的HSP70含量较对照组增加（P<0.01）。与模型组和雷尼替丁治疗组相比，健脾益气方能进一步增加胃溃疡周围黏膜HSP70的表达（P<0.01）。模型组和雷尼替丁治疗组血清及黏膜中HSP70含量较对照组增加（分别为P<0.01，P<0.05）。健脾益气方治疗组血清及黏膜中的HSP70高于模型组和雷尼替丁治疗组（分别为P<0.05，P<0.01）。结论：HSP可能在胃溃疡病理生理过程中发挥作用。健脾益气方能通过HSPs的保护机制促进胃溃疡愈合。
