Yamaguchi H, Osaki T, Taguchi H, Hanawa T, Yamamoto T, Kamiya S
Department of Microbiology, Kyorin University School of Medicine, Tokyo, Japan.
J Gastroenterol. 1996 Nov;31 Suppl 9:12-5.
Induction of heat shock proteins (HSPs) was analyzed in Helicobacter pylori strains. With heat shock at 42 degrees C, a synthesized 60 kDa-HSP (HSP60) was detected on autoradiography. The expression of HSP60 on the cell surface of H. pylori was examined by flow cytometric analysis. All strains used in this study expressed HSP60 on the cell surface, although the intensity differed among the strains, depending on culture conditions. The reactivity of a monoclonal antibody (mAb), 3C8, directed against bacterial HSP60, with HSP60 derived from ten strains of H. pylori and with human gastric carcinoma cell HSP60 was examined by immunoblot analysis. An epitope that reacted with the mAb was detected in the HSP60 of H. pylori and on the surface of human gastric carcinoma cells.
对幽门螺杆菌菌株中热休克蛋白（HSPs）的诱导情况进行了分析。在42℃进行热休克处理后，通过放射自显影检测到一种合成的60 kDa热休克蛋白（HSP60）。采用流式细胞术分析了幽门螺杆菌细胞表面HSP60的表达情况。本研究中使用的所有菌株在细胞表面均表达HSP60，不过根据培养条件不同，各菌株间表达强度存在差异。通过免疫印迹分析检测了一种针对细菌HSP60的单克隆抗体（mAb）3C8与源自十株幽门螺杆菌的HSP60以及人胃癌细胞HSP60的反应性。在幽门螺杆菌的HSP60以及人胃癌细胞表面检测到了与该单克隆抗体发生反应的表位。
