Hessel H, Radon K, Pethran A, Maisch B, Gröbmair S, Sautter I, Fruhmann G
Institute and Outpatient Clinic for Occupational and Environmental Medicine, Ludwig-Maximilians-University, Ziemssenstr. 1, 80336, München, Germany.
Mutat Res. 2001 Oct 18;497(1-2):101-9. doi: 10.1016/s1383-5718(01)00236-4.
The aim of this study was to evaluate the genotoxicity of cytostatic drugs in hospital and pharmacy employees (n=100), occupationally exposed. The micronucleus assay was used to study lymphocytes in 247 peripheral blood samples. Samples were collected at "baseline level" without any cytostatic drugs exposure before recruiting or after at least 3 weeks without cytostatic drugs contact and at three times (cycle 1-3) post-exposure. Samples from 60 office employees served as controls. Furthermore, our results were compared to urinary analyses of cytostatic drugs (oxazaphosporines, anthracyclines, platinum) which were collected in parallel to the cytogenetic investigation. Statistical analyses were performed under consideration of age, gender and X-ray exposure. The frequency of micronuclei was significantly related to the age of the subjects (r(Spearman)=0.16; P<0.05). However, there were no significant differences in micronucleus rates between controls and exposed hospital workers. Similarly, micronucleus rates were not significantly different at the various sampling time points and there was no correlation between duration of employment and micronucleus rates. Furthermore, no correlation between current biomonitoring data of exposure (urine tests) and micronuclei frequency was found. Therefore, significantly increased genotoxic damage of the lymphocytes investigated in this study could not be demonstrated.
本研究旨在评估医院和药房职业暴露的员工（n = 100）接触细胞毒性药物后的遗传毒性。采用微核试验研究247份外周血样本中的淋巴细胞。样本在招募前或至少3周未接触细胞毒性药物后的“基线水平”采集，以及在接触后三个时间点（第1 - 3周期）采集。60名办公室员工的样本作为对照。此外，我们的结果与细胞遗传学调查同时采集的细胞毒性药物（氧氮磷杂环类、蒽环类、铂类）尿液分析结果进行了比较。统计分析考虑了年龄、性别和X射线暴露因素。微核频率与受试者年龄显著相关（斯皮尔曼相关系数r = 0.16；P < 0.05）。然而，对照组和接触药物的医院工作人员之间的微核率没有显著差异。同样，在不同采样时间点微核率也没有显著差异，且就业时长与微核率之间没有相关性。此外，未发现当前接触生物监测数据（尿液检测）与微核频率之间存在相关性。因此，本研究中所调查的淋巴细胞遗传毒性损伤未显著增加。
