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木醋杆菌(=醋酸杆菌)ATCC 10245将葡萄糖胺和N-乙酰葡萄糖胺直接掺入胞外聚合物中:壳聚糖-纤维素和几丁质-纤维素胞外聚合物的生产。

Direct incorporation of glucosamine and N-acetylglucosamine into exopolymers by Gluconacetobacter xylinus (=Acetobacter xylinum) ATCC 10245: production of chitosan-cellulose and chitin-cellulose exopolymers.

作者信息

Lee J W, Deng F, Yeomans W G, Allen A L, Gross R A, Kaplan D L

机构信息

Dong-A University, Hadan 2-dong, Sha-gu, Pusan 604-714, Korea.

出版信息

Appl Environ Microbiol. 2001 Sep;67(9):3970-5. doi: 10.1128/AEM.67.9.3970-3975.2001.

Abstract

Gluconacetobacter xylinus (=Acetobacter xylinum) ATCC 10245 incorporated 2-amino-2-deoxy-D-glucose (glucosamine) and 2-acetamido-2-deoxy-D-glucose (N-acetylglucosamine), but not 3-O-methyl-D-glucose or 2-deoxy-D-glucose into exopolymers. Incorporation was confirmed by gas chromatography with and without mass spectrometry, Fourier transform infrared, and 1H nuclear magnetic resonance. The average molar percentage of glucosamine and N-acetylglucosamine in the exopolymers was about 18%.

摘要

木醋杆菌(=醋化醋杆菌)ATCC 10245将2-氨基-2-脱氧-D-葡萄糖(葡糖胺)和2-乙酰氨基-2-脱氧-D-葡萄糖(N-乙酰葡糖胺)掺入胞外聚合物中,但不掺入3-O-甲基-D-葡萄糖或2-脱氧-D-葡萄糖。通过有无质谱的气相色谱法、傅里叶变换红外光谱法和1H核磁共振法证实了掺入情况。胞外聚合物中葡糖胺和N-乙酰葡糖胺的平均摩尔百分比约为18%。

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