Marschang R E, Gravendyck M, Kaleta E F
Institut für Geflügel- und Reptilienkrankheiten, Justus-Liebig-Universität, Giessen, Germany.
Zentralbl Veterinarmed B. 1997 Sep;44(7):385-94. doi: 10.1111/j.1439-0450.1997.tb00989.x.
Various studies were done during a spontaneous outbreak of stomatitis-rhinitis-complex (mouth rot) in a collection of Mediterranean land tortoises (21 Testudo hermanni, Hermann's tortoises, and three Testudo graeca, spur-thighed tortoises) in southern Germany. These studies were intended to help diagnose the causative agent, establish a possible diagnostic method in vivo and provide information on the efficacy of aciclovir and ganciclovir against chelonian herpesviruses. Thirteen T. hermanni and no T. graeca died within a period of 6 weeks following the introduction of one apparently healthy T. graeca. Two of the dead Testudo hermanni were submitted for post-mortem examination. In addition, blood samples from 11 of the 12 tortoises still surviving at the beginning of this study were cultured for virus content and for the presence of neutralizing antibodies to chelonian herpesviruses and swabs from conjunctiva, pharynx and cloaca were cultured for the presence of viruses. Herpesviruses were isolated from tissues of the two dead Testudo hermanni (tongue, intestine, trachea, lung, spleen, heart and brain). Peripheral leukocytes from one of 11 blood samples were positive for herpesvirus isolation, indicating viremia in at least one animal. Nine of 11 pharyngeal swabs but none of the conjunctival and cloacal swabs yielded herpesviruses. Circulating neutralizing antibodies were present in two of two tested T. graeca, but absent in all of the nine samples from T. hermanni. Aciclovir and ganciclovir were effective when tested in vitro against one of the herpesvirus isolates.
在德国南部一群地中海陆龟（21只赫尔曼陆龟和3只希腊陆龟）自然爆发口炎 - 鼻炎综合征（口腐病）期间，开展了多项研究。这些研究旨在帮助诊断病原体，建立一种可能的体内诊断方法，并提供阿昔洛韦和更昔洛韦对龟疱疹病毒疗效的信息。引入一只看似健康的希腊陆龟后，在6周内有13只赫尔曼陆龟死亡，希腊陆龟无一死亡。对两只死亡的赫尔曼陆龟进行了尸检。此外，对本研究开始时仍存活的12只陆龟中的11只采集血液样本，培养病毒含量以及检测针对龟疱疹病毒的中和抗体，并采集结膜、咽部和泄殖腔拭子培养病毒。从两只死亡的赫尔曼陆龟的组织（舌头、肠道、气管、肺、脾脏、心脏和大脑）中分离出疱疹病毒。11份血液样本中的一份外周血白细胞疱疹病毒分离呈阳性，表明至少有一只动物发生病毒血症。11份咽拭子中有9份检出疱疹病毒，而结膜拭子和泄殖腔拭子均未检出。两只受试希腊陆龟中有两只存在循环中和抗体，但9份赫尔曼陆龟样本均未检出。阿昔洛韦和更昔洛韦在体外对其中一种疱疹病毒分离株检测时有效。
