Polak G, Kozioł-Montewka M, Niedźwiadek J, Tarkowski R, Sidor-Wójtowicz A, Kotarski J
Katedry i Zakładu Mikrobiologii Lekarskiej AM w Lublinie.
Ginekol Pol. 2001 May;72(5):422-6.
To assess the concentrations of tumor necrosis factor alpha (TNF-alpha), interferon gamma (IFN-gamma) and lipid peroxides (the marker of free radicals activity) in peritoneal fluid (PF) of infertile women with minimal and mild endometriosis.
19 women were studied, including 9 infertile women with minimal or mild endometriosis and 10 patients with tubal occlusion (the reference group). Lipid peroxides (malonyldialdehyde and 4-hydroxynonenal), TNF-alpha and IFN-gamma concentrations were measured in the PF using commercially available kits.
Concentration of IFN-gamma was detectable in PF of 7 (77.8%) women with endometriosis and in PF from 3 (30%) patients with tubal occlusion. Neither TNF-alpha or lipid peroxides PF concentration differed significantly (p < 0.05) between the groups. In the group with endometriosis we have found a positive correlation (R = 0.77, p = 0.04) between the concentrations of TNF-alpha and IFN-gamma.
Our results suggest that oxidative stress in the PF doesn't appear to play a role in endometriosis-associated infertility.
评估患有轻度和中度子宫内膜异位症的不孕女性腹腔液中肿瘤坏死因子α（TNF-α）、干扰素γ（IFN-γ）和脂质过氧化物（自由基活性标志物）的浓度。
对19名女性进行了研究，其中包括9名患有轻度或中度子宫内膜异位症的不孕女性以及10名输卵管阻塞患者（参照组）。使用市售试剂盒测定腹腔液中脂质过氧化物（丙二醛和4-羟基壬烯醛）、TNF-α和IFN-γ的浓度。
在7名（77.8%）患有子宫内膜异位症的女性腹腔液以及3名（30%）输卵管阻塞患者的腹腔液中可检测到IFN-γ浓度。两组之间TNF-α或脂质过氧化物腹腔液浓度均无显著差异（p<0.05）。在患有子宫内膜异位症的组中，我们发现TNF-α和IFN-γ浓度之间存在正相关（R=0.77，p=0.04）。
我们的结果表明，腹腔液中的氧化应激似乎在子宫内膜异位症相关不孕中不起作用。
