香石竹环斑病毒衣壳蛋白中的单个氨基酸突变允许病毒粒子形成，但阻止系统感染。
A single amino acid mutation in the carnation ringspot virus capsid protein allows virion formation but prevents systemic infection.
作者信息
Sit T L, Haikal P R, Callaway A S, Lommel S A
机构信息
Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695-7616, USA.
出版信息
J Virol. 2001 Oct;75(19):9538-42. doi: 10.1128/JVI.75.19.9538-9542.2001.
Abstract
A Carnation ringspot virus (CRSV) variant (1.26) was identified that accumulates virions but is incapable of forming a systemic infection. The 1.26 capsid protein gene possesses a Ser-->Pro mutation at amino acid 282. Conversion of 1.26 amino acid 282 to Ser restored systemic infection, while the reciprocal mutation in wild-type CRSV abolished systemic infection. Similar mutations introduced into the related Red clover necrotic mosaic virus capsid protein gene failed to induce the packaging but nonsystemic movement phenotype. These results provide additional support for the theory that virion formation is necessary but not sufficient for systemic movement with the dianthoviruses.
摘要
一种康乃馨环斑病毒（CRSV）变体（1.26）被鉴定出来，它能积累病毒粒子，但无法形成系统性感染。1.26衣壳蛋白基因在氨基酸282处存在丝氨酸（Ser）到脯氨酸（Pro）的突变。将1.26的氨基酸282转换为丝氨酸可恢复系统性感染，而野生型CRSV中的反向突变则消除了系统性感染。在相关的红三叶草坏死花叶病毒衣壳蛋白基因中引入类似突变未能诱导包装但非系统性移动的表型。这些结果为病毒粒子形成对于石竹病毒系统性移动是必要但不充分这一理论提供了额外支持。
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