通过磷酰基和氨基的直接偶联形成核苷酸间3'-5'磷酰胺键。
Formation of internucleotide 3'-5' phosphoramidate links by direct coupling of phosphoryl and amino groups.
作者信息
Greene G L, Letsinger R L
出版信息
Nucleic Acids Res. 1975 Jul;2(7):1123-7. doi: 10.1093/nar/2.7.1123.
Abstract
The stepwise synthesis of oligomers derived from 5'-amino-5'-deoxythymidine 3'-phosphate and 5'-amino-5'-deoxy-3'-O-mono-p-methoxytrityl-thymidine is described. The internucleotide phosphoramidate links were formed by condensation of nucleoside 3'-phosphates with aminonucleosides by means of triphenylphosphine and dipridyl disulfide.
摘要
描述了由5'-氨基-5'-脱氧胸苷3'-磷酸酯和5'-氨基-5'-脱氧-3'-O-单对甲氧基三苯甲基胸苷逐步合成低聚物的过程。核苷酸间的磷酰胺键是通过核苷3'-磷酸酯与氨基核苷借助三苯基膦和联吡啶二硫化物缩合形成的。
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Use of phosphorus oxychloride in synthesizing nucleotides and oligonucleotides.三氯氧磷在核苷酸和寡核苷酸合成中的应用。
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2
Phosphoramidate analogs of oligonucleotides.寡核苷酸的磷酰胺酯类似物。
J Org Chem. 1970 Nov;35(11):3800-3. doi: 10.1021/jo00836a048.
3
Use of trityl- and -naphthylcarbamoylcellulose derivatives in oligonucleotide synthesis.三苯甲基和萘基氨基甲酰纤维素衍生物在寡核苷酸合成中的应用。
Biochemistry. 1973 May 8;12(10):1985-90. doi: 10.1021/bi00734a023.
4
Synthesis of oligothymidylates and nucleoside cyclic phosphates by oxidation-reduction condensation.通过氧化还原缩合合成寡聚胸苷酸和核苷环磷酸酯。
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5
Enzymatic synthesis of polydeoxyribonucleotides possessing internucleotide phosphoramidate bonds.具有核苷酸间磷酰胺键的聚脱氧核糖核苷酸的酶促合成。
J Am Chem Soc. 1972 Jan 12;94(1):292-3. doi: 10.1021/ja00756a060.
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