Dhoot G K, Gustafsson M K, Ai X, Sun W, Standiford D M, Emerson C P
Department of Basic Veterinary Sciences, The Royal Veterinary College, University of London, Royal College Street, London NW1 OTU, UK.
Science. 2001 Aug 31;293(5535):1663-6. doi: 10.1126/science.293.5535.1663.
The developmental signaling functions of cell surface heparan sulfate proteoglycans (HSPGs) are dependent on their sulfation states. Here, we report the identification of QSulf1, the avian ortholog of an evolutionarily conserved protein family related to heparan-specific N-acetyl glucosamine sulfatases. QSulf1 expression is induced by Sonic hedgehog in myogenic somite progenitors in quail embryos and is required for the activation of MyoD, a Wnt-induced regulator of muscle specification. QSulf1 is localized on the cell surface and regulates heparan-dependent Wnt signaling in C2C12 myogenic progenitor cells through a mechanism that requires its catalytic activity, providing evidence that QSulf1 regulates Wnt signaling through desulfation of cell surface HSPGs.
细胞表面硫酸乙酰肝素蛋白聚糖（HSPGs）的发育信号功能取决于其硫酸化状态。在此，我们报告了QSulf1的鉴定，它是与硫酸乙酰肝素特异性N-乙酰葡糖胺硫酸酯酶相关的进化保守蛋白家族的禽类直系同源物。QSulf1的表达由鹌鹑胚胎成肌体节祖细胞中的音猬因子诱导，是激活MyoD（一种Wnt诱导的肌肉特化调节因子）所必需的。QSulf1定位于细胞表面，并通过一种需要其催化活性的机制调节C2C12成肌祖细胞中依赖硫酸乙酰肝素的Wnt信号，这证明QSulf1通过细胞表面HSPGs的去硫酸化来调节Wnt信号。
