Dultsev F N, Speight R E, Fiorini M T, Blackburn J M, Abell C, Ostanin V P, Klenerman D
Department of Chemistry, Cambridge University, UK.
Anal Chem. 2001 Aug 15;73(16):3935-9. doi: 10.1021/ac0100897.
We show that it is possible to detect specifically adsorbed bacteriophage directly by breaking the interactions between proteins displayed on the phage coat and ligands immobilized on the surface of a quartz crystal microbalance (QCM). This is achieved through increasing the amplitude of oscillation of the QCM surface and sensitively detecting the acoustic emission produced when the bacteriophage detaches from the surface. There is no interference from nonspecifically adsorbed phage. The detection is quantitative over at least 5 orders of magnitude and is sensitive enough to detect as few as 20 phage. The method has potential as a sensitive and low-cost method for virus detection.
我们表明，通过打破噬菌体衣壳上展示的蛋白质与固定在石英晶体微天平（QCM）表面的配体之间的相互作用，可以直接检测特异性吸附的噬菌体。这是通过增加QCM表面的振荡幅度并灵敏地检测噬菌体从表面脱离时产生的声发射来实现的。不存在非特异性吸附噬菌体的干扰。该检测在至少5个数量级上是定量的，并且灵敏度足以检测低至20个噬菌体。该方法有潜力成为一种灵敏且低成本的病毒检测方法。
