Cooper M A, Dultsev F N, Minson T, Ostanin V P, Abell C, Klenerman D
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, United Kingdom.
Nat Biotechnol. 2001 Sep;19(9):833-7. doi: 10.1038/nbt0901-833.
We have developed a sensitive, economical method that directly detects viruses by making use of the interaction between type 1 herpes simplex virus (HSV1) and specific antibodies covalently attached to the oscillating surface of a quartz crystal microbalance (QCM). The virions were detached from the surface by monotonously increasing the amplitude of oscillation of the QCM, while using the QCM to sensitively detect the acoustic noise produced when the interactions were broken. We term this process rupture event scanning (REVS). The method is quantitative over at least six orders of magnitude, and its sensitivity approaches detection of a single virus particle.
我们开发了一种灵敏且经济的方法，通过利用1型单纯疱疹病毒（HSV1）与共价附着在石英晶体微天平（QCM）振荡表面的特异性抗体之间的相互作用来直接检测病毒。通过单调增加QCM的振荡幅度，使病毒粒子从表面脱离，同时利用QCM灵敏地检测相互作用被破坏时产生的声学噪声。我们将这个过程称为破裂事件扫描（REVS）。该方法在至少六个数量级上是定量的，其灵敏度接近检测单个病毒颗粒。
