Mansour M, Lanphear B P, Hornung R, Khoury J, Bernstein D I, Menrath W, Decolongon J
Cincinnati Lead and Allergen Sampling Study with Children's Hospital Medical Center, Department of Pediatrics, Ohio 45229, USA.
Environ Res. 2001 Sep;87(1):37-46. doi: 10.1006/enrs.2001.4284.
Exposure to indoor allergens is associated with asthma, but there is no standardized sampling method for measuring allergens. We compared the association of measured allergen exposure and serum-specific IgE levels and the precision of three sampling methods (Cyclone, Mighty Mite, and Readivac II) to identify a standardized sampling method for indoor allergens. A random sample of 72 children, 5 to 17 years old, with doctor-diagnosed asthma who lived in the same residence >or=2 years were enrolled. Composite, side by side floor samples were obtained with all three methods. Dust allergen concentrations and serum-specific IgE levels were measured for Der f I, Fel d I, and Bla g I. Mean allergen concentration did not differ significantly by sampling method. Cat allergen was significantly correlated with serum-specific IgE for Cyclone (P=0.003) and Mighty Mite (P=0.008), but only marginally for Readivac II (P=0.07). Dust mite allergen was significantly correlated with serum-specific IgE for Readivac II (P=0.02) and Cyclone (P=0.038), but not for Mighty Mite (P=0.12). Cockroach allergen was not correlated with serum-specific IgE for any sampling method. In multiple linear regression, cat allergen was associated with serum-specific IgE for Cyclone (P=0.007) and Mighty Mite (P=0.02), but not for Readivac II (P=0.06). In contrast, dust mite allergen was marginally associated with serum-specific IgE for Readivac II (P=0.07), but not for Mighty Mite (P=0.64) or Cyclone (P=0.27). The Cyclone and Mighty Mite were more precise than Readivac II for cat allergen, but there was no difference for dust mite allergen (P>0.05). No single method is superior for measurement of indoor allergens. In general, cat allergen collected with the Cyclone was a better predictor of serum-specific IgE levels to Fel d I, whereas dust mite allergen collected with the Readivac II was a better predictor of serum-specific IgE levels to Der f I.
接触室内过敏原与哮喘有关，但目前尚无用于测量过敏原的标准化采样方法。我们比较了测量的过敏原暴露与血清特异性IgE水平之间的关联，以及三种采样方法（Cyclone、Mighty Mite和Readivac II）的精确度，以确定一种用于室内过敏原的标准化采样方法。随机抽取了72名5至17岁、经医生诊断患有哮喘且在同一住所居住≥2年的儿童。使用这三种方法获取了并排的复合地板样本。测量了粉尘螨Der f I、猫Fel d I和蟑螂Bla g I的过敏原浓度及血清特异性IgE水平。平均过敏原浓度在不同采样方法之间无显著差异。猫过敏原与Cyclone（P = 0.003）和Mighty Mite（P = 0.008）的血清特异性IgE显著相关，但与Readivac II仅呈边缘相关（P = 0.07）。粉尘螨过敏原与Readivac II（P = 0.02）和Cyclone（P = 0.038）的血清特异性IgE显著相关，但与Mighty Mite不相关（P = 0.12）。蟑螂过敏原在任何采样方法中均与血清特异性IgE不相关。在多元线性回归中，猫过敏原与Cyclone（P = 0.007）和Mighty Mite（P = 0.02）的血清特异性IgE相关，但与Readivac II不相关（P = 0.06）。相比之下，粉尘螨过敏原与Readivac II的血清特异性IgE呈边缘相关（P = 0.07），但与Mighty Mite（P = 0.64）或Cyclone（P = 0.27）不相关。对于猫过敏原，Cyclone和Mighty Mite比Readivac II更精确，但对于粉尘螨过敏原则无差异（P>0.05）。在测量室内过敏原方面，没有一种方法是更优越的。一般来说，用Cyclone采集的猫过敏原是血清对Fel d I特异性IgE水平的更好预测指标，而用Readivac II采集的粉尘螨过敏原是血清对Der f I特异性IgE水平的更好预测指标。
