Children's Mercy Center for Environmental Health, Children's Mercy Hospitals and Clinics, Kansas City, MO 64108, USA.
Ann Allergy Asthma Immunol. 2013 May;110(5):375-9. doi: 10.1016/j.anai.2013.02.010. Epub 2013 Mar 7.
Assessment of indoor allergen is valuable in exposure research and evaluation of allergic individuals. Collection methods range from grab vacuum samples to filtration devices located in the breathing range of an individual. For practical purposes, many research studies use analysis of collected house dust to evaluate allergen reservoirs.
To test the hypothesis that house dust collected from the family vacuum is equivalent to house dust collected by a technician following standard protocol.
Homes from a healthy homes demonstration project (n = 41) were sampled using a specific Department of Housing and Urban Development-suggested protocol in the bedroom of the child with asthma and a simple grab procedure from the family vacuum. Samples were evaluated for the presence of 5 allergens, Bla g2, Can f1, Der f1, and Der p1 combined as total mite, Fel d1, and Mus m1. Samples were also evaluated for total antigenic protein from 4 fungal taxa, including Alternaria, Aspergillus, Cladosporium, and Penicillium.
All of the allergens and antigens tested showed good correlation between the 2 collection methods. Fungal antigens ranged up to 92,651 nanograms per gram of dust for Aspergillus, and allergens ranged up to 17,928 nanograms per gram of dust for Can f1. The best correlation was for Cladosporium (r = 0.91), and the weakest was for dust mite (r = 0.34).
Allergens and antigens tested from samples collected by protocol and by grab sampling from the home vacuum were highly positively correlated. Grab samples taken from the family vacuum may be a good surrogate for evaluating home allergen exposure.
室内过敏原评估在暴露研究和评估过敏个体方面具有重要价值。收集方法从抓握式真空取样到位于个人呼吸范围内的过滤设备不等。出于实际目的，许多研究使用收集的室内灰尘分析来评估过敏原库。
检验以下假设，即从家庭真空吸尘器中收集的室内灰尘与按照标准协议由技术人员收集的室内灰尘等同。
对健康住宅示范项目（n=41）中的房屋进行采样，在患有哮喘的儿童的卧室中采用特定的住房和城市发展部建议的协议以及从家庭真空吸尘器中进行简单的抓握式取样。评估 5 种过敏原（Bla g2、Can f1、Der f1 和 Der p1 组合为总螨、Fel d1 和 Mus m1）、4 种真菌类群（包括链格孢属、曲霉菌属、枝孢菌属和青霉菌属）的总抗原蛋白。
所有测试的过敏原和抗原均显示出这两种收集方法之间的良好相关性。真菌抗原的范围高达 92651 纳克/克灰尘（曲霉菌），过敏原的范围高达 17928 纳克/克灰尘（Can f1）。相关性最好的是枝孢菌（r=0.91），而尘螨相关性最差（r=0.34）。
通过协议收集的样本和通过家庭真空吸尘器的抓握式取样测试的过敏原和抗原具有高度正相关性。从家庭真空吸尘器中采集的抓握式样本可能是评估家庭过敏原暴露的良好替代品。
