吸入性炭疽兔模型中免疫的体外相关性
In vitro correlate of immunity in a rabbit model of inhalational anthrax.
作者信息
Pitt M L, Little S F, Ivins B E, Fellows P, Barth J, Hewetson J, Gibbs P, Dertzbaugh M, Friedlander A M
机构信息
United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.
出版信息
Vaccine. 2001 Sep 14;19(32):4768-73. doi: 10.1016/s0264-410x(01)00234-1.
A serological correlate of vaccine-induced immunity was identified in the rabbit model of inhalational anthrax. Animals were inoculated intramuscularly at 0 and 4 weeks with varying doses of Anthrax Vaccine Adsorbed (AVA) ranging from a human dose to a 1:256 dilution in phosphate-buffered saline (PBS). At 6 and 10 weeks, both the quantitative anti-protective antigen (PA) IgG ELISA and the toxin-neutralizing antibody (TNA) assays were used to measure antibody levels to PA. Rabbits were aerosol-challenged at 10 weeks with a lethal dose (84-133 LD(50)) of Bacillus anthracis spores. All the rabbits that received the undiluted and 1:4 dilution of vaccine survived, whereas those receiving the higher dilutions of vaccine (1:16, 1:64 and 1:256) had deaths in their groups. Results showed that antibody levels to PA at both 6 and 10 weeks were significant (P<0.0001) predictors of survival.
在吸入性炭疽的兔模型中确定了疫苗诱导免疫的血清学关联物。在第0周和第4周，给动物肌肉注射不同剂量的吸附炭疽疫苗（AVA），剂量范围从人用剂量到在磷酸盐缓冲盐水（PBS）中1:256的稀释液。在第6周和第10周，使用定量抗保护性抗原（PA）IgG ELISA和毒素中和抗体（TNA）测定法来测量针对PA的抗体水平。在第10周，用致死剂量（84 - 133 LD(50)）的炭疽芽孢杆菌孢子对兔子进行气溶胶攻击。所有接受未稀释疫苗和1:4稀释疫苗的兔子存活，而接受更高稀释度疫苗（1:16、1:64和1:256）的组中有兔子死亡。结果表明，第6周和第10周时针对PA的抗体水平是存活的显著（P<0.0001）预测指标。
Zotero 插件