手术可诱导人单核细胞精氨酸酶I的表达。
Surgery induces human mononuclear cell arginase I expression.
作者信息
Tsuei B J, Bernard A C, Shane M D, Shirley L A, Maley M E, Boulanger B R, Kearney P A, Ochoa J B
机构信息
Department of Surgery, Vascular and Trauma/Critical Care Research Program, University of Kentucky College of Medicine, 800 Rose Street, Lexington, KY 40536, USA.
出版信息
J Trauma. 2001 Sep;51(3):497-502. doi: 10.1097/00005373-200109000-00012.
BACKGROUND
Arginase is a metabolic enzyme for the amino acid arginine that participates in the immune response to trauma. We hypothesize that surgical trauma induces arginase expression and activity in the human immune system.
METHODS
Peripheral mononuclear cell (MNC) arginase activity and expression and plasma nitric oxide metabolites and interleukin (IL)-10 were measured in patients undergoing elective general surgery. Twenty-two healthy volunteers served as a comparison population.
RESULTS
MNC arginase activity increased within 6 hours of surgery (p < 0.05) and coincided with increased arginase I protein expression. Plasma nitric oxide metabolites decreased significantly postoperatively (p < 0.05). Patients lacking an elevation in IL-10 failed to demonstrate increased MNC arginase activity.
CONCLUSION
Increased MNC arginase expression may contribute to postsurgical immune dysfunction by affecting arginine use and availability and nitric oxide metabolism in the immune system. Plasma IL-10 may play a role in regulating MNC arginase activity.
背景
精氨酸酶是一种参与创伤免疫反应的氨基酸精氨酸代谢酶。我们假设手术创伤会诱导人体免疫系统中精氨酸酶的表达和活性。
方法
对接受择期普通外科手术的患者测量外周血单个核细胞（MNC）精氨酸酶活性、表达以及血浆一氧化氮代谢产物和白细胞介素（IL）-10水平。22名健康志愿者作为对照人群。
结果
手术6小时内MNC精氨酸酶活性增加（p < 0.05），且与精氨酸酶I蛋白表达增加一致。术后血浆一氧化氮代谢产物显著降低（p < 0.05）。IL-10未升高的患者未表现出MNC精氨酸酶活性增加。
结论
MNC精氨酸酶表达增加可能通过影响免疫系统中精氨酸的利用和可用性以及一氧化氮代谢，导致术后免疫功能障碍。血浆IL-10可能在调节MNC精氨酸酶活性中发挥作用。
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