Pioruńska-Stolzmann M, Iskra M, Majewski W
Department of General Chemistry, Chair and Department of Clinical Biochemistry, Medical University, Poznań, Poland.
Med Sci Monit. 2001 Sep-Oct;7(5):940-5.
Ceruloplasmin is a copper-containing (2-glycoprotein and a member of the acute phase reactant family. Parallel changes in ceruloplasmin and copper concentration have been observed in diseases accompanied by severe inflammation, indicating a closely related process. Cholesterol esterase participates in lipoprotein degradation by the hydrolysis of cholesteryl esters in low density lipoproteins (LDL-CE). LDL-CEs are substrates for cholesterol esterase, but can also undergo oxidation by metal ions. The reduction of lipid hydroperoxides has been claimed to play a key role in the control of lipid peroxidation in living systems.
The experimental group consisted of 16 men with atherosclerosis obliterans of the lower limbs, with 12 healthy male volunteers as a control group. The activity of cholesterol esterase (CEase), ceruloplasmin (Cp), and glutathione peroxidase (GPx), and the concentrations of malonyldialdehyde (MDA) and copper were determined in serum samples.
CEase and Cp activity and copper concentrations were found to be greater in the AO group than in the controls. In addition, CEase and Cp were negatively correlated (r=(0.65, p< or = 0.05) in the serum of men with AO, but not in the controls. Moreover, copper concentration was significantly correlated with the activity of Cp and GPx (r=0.81 and r=0.58, respectively). The reduced GPx activity and the higher MDA concentration found in the AO group indicate a decline in the antioxidative barrier of plasma.
The results reported here show an inverse relationship between the activities of CEase and Cp in chronic arterial occlusion of the lower limbs, which may reveal the interaction between the antioxidant and lipolytic enzymes.
铜蓝蛋白是一种含铜的α2-糖蛋白，属于急性期反应物家族。在伴有严重炎症的疾病中，已观察到铜蓝蛋白和铜浓度的平行变化，表明这是一个密切相关的过程。胆固醇酯酶通过水解低密度脂蛋白中的胆固醇酯（LDL-CE）参与脂蛋白降解。LDL-CE是胆固醇酯酶的底物，但也可被金属离子氧化。脂质氢过氧化物的还原被认为在生物系统中脂质过氧化的控制中起关键作用。
实验组由16名下肢闭塞性动脉粥样硬化男性患者组成，以12名健康男性志愿者作为对照组。测定血清样本中胆固醇酯酶（CEase）、铜蓝蛋白（Cp）和谷胱甘肽过氧化物酶（GPx）的活性，以及丙二醛（MDA）和铜的浓度。
发现闭塞性动脉粥样硬化（AO）组的CEase和Cp活性以及铜浓度高于对照组。此外，在AO男性患者的血清中，CEase和Cp呈负相关（r = -0.65，p≤0.05），而在对照组中则无此相关性。此外，铜浓度与Cp和GPx的活性显著相关（分别为r = 0.81和r = 0.58）。AO组中GPx活性降低和MDA浓度升高表明血浆抗氧化屏障下降。
本文报道的结果显示，下肢慢性动脉闭塞时CEase和Cp的活性呈负相关，这可能揭示了抗氧化酶和脂解酶之间的相互作用。
