Majewski W, Iskra M, Stanisic M, Laciak M, Mackiewicz A, Staniszewski R
Department of General and Vascular Surgery, Poznan University of Medical Sciences, Poznan, Poland.
Int Angiol. 2007 Dec;26(4):341-5.
Inflammatory reactions accompanying ischemia increase the cytokine synthesis what leads to the increase in serum ceruloplasmin (Cp) concentration and activity. The aim of the study was to evaluate the association between serum Cp oxidase concentration and activity and the grade of lower extremity ischemia. Moreover, the correlation of Cp concentration and activity with the levels of interleukin 6 (IL-6), C-reacting protein (CRP), and a-1-acid glycoprotein (AGP) in serum was studied.
Two groups of patients were examined: 15 patients with moderate (MI) and 32 patients with critical ischemia (CI) of the lower extremities. Cp oxidase activity was measured spectrophotometrically, after incubation with o-dianisidine. The concentration of IL-6 was measured with the ELISA method, and Cp, CRP and AGP concentration by rocket immunoelectrophoresis.
Significant increase in Cp oxidase concentration and activity was observed in patients with critical limb ischemia (median: 164.8 U/L), especially in patients with necrotic changes (median: 216.6 U/L). Cp oxidase activity was dependent on its concentration in patients with critical limb ischemia with necrotic changes (r=0.56; P<0.01). In patients with critical limb ischemia, the increase in Cp concentration and activity correlated significantly with CRP concentration (r=0.46; P=0.0007) (r=0.62; P=0.0001), respectively.
Concentration and the oxidase activity of Cp depend of the degree of lower extremity ischemia and correlates with the major markers of inflammation, such as CRP. Critical limb ischemia induces the inflammatory reaction triggering the increase in IL-6 and of acute phase protein production. These processes lead to the increase in Cp oxidase activity dependent of Cp and CRP concentration.
缺血伴随的炎症反应会增加细胞因子的合成，进而导致血清铜蓝蛋白（Cp）浓度和活性升高。本研究旨在评估血清Cp氧化酶浓度和活性与下肢缺血程度之间的关联。此外，还研究了Cp浓度和活性与血清白细胞介素6（IL-6）、C反应蛋白（CRP）和α-1-酸性糖蛋白（AGP）水平的相关性。
对两组患者进行了检查：15例中度下肢缺血（MI）患者和32例重度下肢缺血（CI）患者。与邻联茴香胺孵育后，用分光光度法测量Cp氧化酶活性。用酶联免疫吸附测定法测量IL-6的浓度，用火箭免疫电泳法测量Cp、CRP和AGP的浓度。
在重度肢体缺血患者中观察到Cp氧化酶浓度和活性显著升高（中位数：164.8 U/L），尤其是有坏死改变的患者（中位数：216.6 U/L）。在有坏死改变的重度肢体缺血患者中，Cp氧化酶活性依赖于其浓度（r = 0.56；P < 0.01）。在重度肢体缺血患者中，Cp浓度和活性的升高分别与CRP浓度显著相关（r = 0.46；P = 0.0007）（r = 0.62；P = 0.0001）。
Cp的浓度和氧化酶活性取决于下肢缺血的程度，并与炎症的主要标志物如CRP相关。重度肢体缺血会引发炎症反应，导致IL-6增加和急性期蛋白产生。这些过程导致Cp氧化酶活性升高，且该活性依赖于Cp和CRP浓度。
