Iskra M, Majewski W
Department of General Chemistry, Karol Marcinkowski University of Medical Sciences, Poznan, Poland.
J Trace Elem Med Biol. 1999 Jul;13(1-2):76-81. doi: 10.1016/S0946-672X(99)80027-3.
The presence of ischaemic tissue excites an inflammatory reaction and synthesis of acute phase proteins (APhPs). Ceruloplasmin (Cp) protein binds 90% of the copper in plasma and it is one of the positive APhPs, and its concentration increases in infection, inflammation or necrosis. The study presents the relationship of the oxidase activity of Cp and concentrations of Cu and Zn in serum of men with different degrees of ischaemia of the lower limbs. The subjects were 32 men with chronic arterial occlusion (AO) of the lower limbs. The oxidase activity of Cp was measured in serum with o-dianisidine as a substrate. Concentrations of Cu and Zn were determined by using atomic absorption spectrometry. The mean activity of Cp in serum in AO (173 +/- 69.2 U/l) was higher as compared with the control group (123.7 +/- 28.6 U/l), and in men with critical ischaemia (> or = 194.8 U/l) than in men with a moderate level of ischaemia (109.3 +/- 31.6 U/l). The mean concentrations of Cu and Zn in serum were found to be higher in AO (22.2 +/- 4.2 and 19.1 +/- 6.9 mumol/l, respectively) than in the control group (16.3 +/- 1.8 and 15.2 +/- 2.3 mumol/l), and in men with critical ischaemia (> or = 22.2 and 19.1 mumol/l) than in men with a moderate level of ischaemia (18.5 +/- 3.3 and 14.5 +/- 4.3 mumol/l). Significant positive correlation coefficients were calculated for the activities of Cp and concentrations of Cu in the control group (r = 0.86) and the AO group (r = 0.76), and low, but significant, correlations for Cp and Zn in the AO group (r = 0.66). The increase in the oxidase activity of Cp and concentration of Cu in serum in ischaemia is caused by the acute phase response. The relationship of Zn concentration and Cp activity in ischaemia is indirect and needs further study.
缺血组织的存在会引发炎症反应和急性期蛋白（APhPs）的合成。铜蓝蛋白（Cp）可结合血浆中90%的铜，它是一种阳性急性期蛋白，其浓度在感染、炎症或坏死时会升高。本研究呈现了不同程度下肢缺血男性血清中Cp的氧化酶活性与铜和锌浓度之间的关系。研究对象为32例患有下肢慢性动脉闭塞（AO）的男性。以邻联茴香胺为底物测定血清中Cp的氧化酶活性。采用原子吸收光谱法测定铜和锌的浓度。AO患者血清中Cp的平均活性（173±69.2 U/l）高于对照组（123.7±28.6 U/l），重度缺血患者（≥194.8 U/l）高于中度缺血患者（109.3±31.6 U/l）。发现AO患者血清中铜和锌的平均浓度（分别为22.2±4.2和19.1±6.9 μmol/l）高于对照组（16.3±1.8和15.2±2.3 μmol/l），重度缺血患者（≥22.2和19.1 μmol/l）高于中度缺血患者（18.5±3.3和14.5±4.3 μmol/l）。在对照组（r = 0.86）和AO组（r = 0.76）中，计算出Cp活性与铜浓度之间存在显著正相关系数，在AO组中，Cp与锌之间存在低但显著的相关性（r = 0.66）。缺血时血清中Cp氧化酶活性和铜浓度的升高是由急性期反应引起的。缺血时锌浓度与Cp活性之间的关系是间接的，需要进一步研究。
