仓鼠海马切片制备中神经活动的温度依赖性。

Thermal dependence of neural activity in the hamster hippocampal slice preparation.

作者信息

Horowitz J M, Thomas M P, Eckerman P

机构信息

Department of Animal Physiology, University of California, Davis 95616, USA.

出版信息

J Therm Biol. 1987;12(2):97-101. doi: 10.1016/0306-4565(87)90045-3.

DOI:10.1016/0306-4565(87)90045-3

Abstract

Neural activity was recorded in an in vitro hamster hippocampal slice preparation while the temperature of the Ringer's solution bathing in the slice was controlled at selected levels. 2. The amplitude of the population spike (action potentials from a group of pyramidal cells) was measured as bath temperature was lowered from 35 degrees C to temperatures where a response could not be evoked. 3. Plots of population spike amplitude versus temperature have bell-shaped curves. The population spikes increased in amplitude as temperature was lowered from 35 degrees C, reached a peak amplitude between 25 and 20 degrees C, and then decreased until a response could not be evoked when temperature was further lowered. 4. These in vitro results obtained in the slice preparation are related to in vivo hippocampal studies. Results are interpreted as consistent with the proposal reviewed here that neural activity in the hippocampus plays a role at specific stages of entrance into and arousal from hibernation.

摘要

在体外仓鼠海马切片标本中记录神经活动，同时将浸泡切片的林格氏液温度控制在选定水平。2. 当浴温从35摄氏度降低到无法诱发反应的温度时，测量群体峰电位（一组锥体细胞的动作电位）的幅度。3. 群体峰电位幅度与温度的关系图呈钟形曲线。随着温度从35摄氏度降低，群体峰电位幅度增加，在25至20摄氏度之间达到峰值幅度，然后随着温度进一步降低而减小，直至无法诱发反应。4. 在切片标本中获得的这些体外实验结果与体内海马研究相关。结果被解释为与本文所综述的观点一致，即海马体中的神经活动在进入冬眠和从冬眠中苏醒的特定阶段发挥作用。