Alexandrova K S, Denchev P D, Conger B V
The University of Tennessee, Dep. of Plant and Soil Science, Knoxville 37901-1071, USA.
Crop Sci. 1996 Nov-Dec;36(6):1709-11. doi: 10.2135/cropsci1996.0011183x003600060049x.
Switchgrass (Panicum virgatum L.) is naturally outcrossing; therefore, maintenance of genotype is difficult through sexual propagation. The objective of this study was to develop a micropropagation procedure for the multiplication of desired or selected genotypes. Nodal segments were surface sterilized, split longitudinally, and placed with the cut surface on solid MS medium containing 30 g L-1 maltose and different concentrations of 6-benzylaminopurine (BAP) as the only growth regulator. Best shoot proliferation was obtained with 12.5 micromoles BAP and culture at 29 degrees C. Shoots were easily rooted on MS medium without BAP. Under optimum conditions, it is possible to produce approximately 500 plantlets from one parent plant in 12 wk.
柳枝稷（Panicum virgatum L.）是天然异花授粉植物；因此，通过有性繁殖维持基因型很困难。本研究的目的是开发一种微繁殖程序，用于繁殖所需或选定的基因型。将茎节进行表面消毒，纵向切开，然后将切面放置在含有30 g L-1麦芽糖和不同浓度6-苄基腺嘌呤（BAP）作为唯一生长调节剂的固体MS培养基上。使用12.5微摩尔BAP并在29摄氏度下培养可获得最佳的芽增殖效果。芽在不含BAP的MS培养基上很容易生根。在最佳条件下，12周内从一株亲本植物中大约可以产生500株幼苗。
