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鼠李糖生物合成途径为多杀菌素链霉菌的初级和次级代谢提供前体。

Rhamnose biosynthesis pathway supplies precursors for primary and secondary metabolism in Saccharopolyspora spinosa.

作者信息

Madduri K, Waldron C, Merlo D J

机构信息

Dow AgroSciences LLC, Indianapolis, Indiana 46268, USA.

出版信息

J Bacteriol. 2001 Oct;183(19):5632-8. doi: 10.1128/JB.183.19.5632-5638.2001.

DOI:10.1128/JB.183.19.5632-5638.2001
PMID:11544225
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC95454/
Abstract

Rhamnose is an essential component of the insect control agent spinosad. However, the genes coding for the four enzymes involved in rhamnose biosynthesis in Saccharopolyspora spinosa are located in three different regions of the genome, all unlinked to the cluster of other genes that are required for spinosyn biosynthesis. Disruption of any of the rhamnose genes resulted in mutants with highly fragmented mycelia that could survive only in media supplemented with an osmotic stabilizer. It appears that this single set of genes provides rhamnose for cell wall synthesis as well as for secondary metabolite production. Duplicating the first two genes of the pathway caused a significant improvement in the yield of spinosyn fermentation products.

摘要

鼠李糖是杀虫剂多杀菌素的一种重要成分。然而，编码参与多刺糖多孢菌鼠李糖生物合成的四种酶的基因位于基因组的三个不同区域，均与多杀菌素生物合成所需的其他基因簇不连锁。任何一个鼠李糖基因的破坏都会导致突变体的菌丝体高度碎片化，这些突变体只能在添加了渗透稳定剂的培养基中存活。看来这一组基因既为细胞壁合成也为次级代谢产物生产提供鼠李糖。复制该途径的前两个基因导致多杀菌素发酵产物的产量显著提高。

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Cloning and analysis of the spinosad biosynthetic gene cluster of Saccharopolyspora spinosa.多刺糖多孢菌多杀菌素生物合成基因簇的克隆与分析。
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