Pushkarev V P, Novikov P I
Sud Med Ekspert. 2001 Mar-Apr;44(2):21-6.
A series of screening tests showed that separation of polymerase chain reaction products and identification of D1S80 by capillary electrophoresis with the use of hydroxyethyl cellulose as the substituting screening template and double internal DNA reference is an accurate, rapid, and reliable method, fit for routine use. D1S80-allele frequencies for Caucasian population were determined in a population of 340 unrelated individuals living in the Ural region of Russia. The D1S80 genotypical frequencies in this population sample do not statistically deviate from the Hardy-Weinberg expectations. The discrimination power for this locus is 0.935. Significant deviations from the zero hypothesis of population homogeneity were detected by comparison of 4 Caucasian population samples. Differences in the frequencies of alleles 25, 30, and 31 seem to be the main cause of heterogeneity in paired comparison.
一系列筛选试验表明，以羟乙基纤维素作为替代筛选模板并使用双重内部DNA参照，通过毛细管电泳分离聚合酶链反应产物并鉴定D1S80，是一种准确、快速且可靠的方法，适用于常规使用。在居住于俄罗斯乌拉尔地区的340名无关个体组成的群体中测定了高加索人群的D1S80等位基因频率。该群体样本中的D1S80基因型频率在统计学上与哈迪-温伯格预期无偏差。该位点的鉴别力为0.935。通过比较4个高加索人群样本，检测到与群体同质性零假设存在显著偏差。在配对比较中，等位基因25、30和31频率的差异似乎是异质性的主要原因。
