Huttunen R
Acta Chir Scand. 1975;141(4):285-8.
Precursors of proteolytic enzymes were demonstrated in the peritoneal inflammatory exudate during acute experimental pancreatitis of the rat. This was done by separating the proteinase inhibitors and proenzymes by gel filtration on Sephadex G-200. After elution the proenzymes could be demonstrated by activating them with enterokinase or with trypsin. The proenzymes were eluted after the main protein bulk and proteinase inhibitors. Enzyme precursors were absent from the exudate of formalin-induced peritonitis, which suggests that the proenzymes present in the exudate of pancreatitis are of pancreatic origin. The demonstration of proenzymes in perripheral blood during pancreatitis was tested with the several modifications of the same methods, but the results were not convincing, probably owing to the insensitivity of the methods used.
在大鼠急性实验性胰腺炎期间，在腹膜炎性渗出物中证实了蛋白水解酶的前体。这是通过在葡聚糖凝胶G - 200上进行凝胶过滤来分离蛋白酶抑制剂和酶原实现的。洗脱后，可通过肠激酶或胰蛋白酶激活酶原以证实其存在。酶原在主要蛋白质和蛋白酶抑制剂之后被洗脱。福尔马林诱导的腹膜炎渗出物中不存在酶前体，这表明胰腺炎渗出物中存在的酶原来源于胰腺。用相同方法的几种改良方法对胰腺炎期间外周血中的酶原进行了检测，但结果并不令人信服，可能是由于所用方法不够灵敏。
