Huttunen R
Scand J Gastroenterol. 1975;10(2):177-80.
The trypsinogen and chymotrypsinogen contents of the pancreas were examined during acute experimental pacreatitiis of the rat. The proenzymes were activated with enterokinase and the amounts of active proteases were estimated with BAPNA (N-alfa-benzoyl-DL-arginin-4-nitroanilid hydrochlorid, Fluka AG) and SUPHEPA (succinyl-L-phenylalanine-p-nitroanilide, Schwarz/Mann, Division of Becton) as the substrates. The activation of chymotrypsinogen was more rapid than the activation of trypsinogen; maximal activation occurred in 3 hours. Under similar circumstances the activation of trypsinogen required 17 hours. Both trypsinogen and chymotrypsinogen content decreased significantly during the inflammation. In 8 hours the decline of trypsinogen content was 28.4 percent and that of chymotrypsinogen content 44.9 percent from the proenzyme content of the normal resting rat pancreas. This indicates that proenzymes and/or active proteases are liberated during the course of pancreatitis. No correlation was found between the trypsinogen and the chymotrypsinogen content of the normal pancreas, but during pancreatitis the proenzyme contents correlated clearly. The correlation during inflammation possibly reflects the amount of the viable pancreatic tissue and the rate of synthesis.
在大鼠急性实验性胰腺炎期间，对胰腺中的胰蛋白酶原和糜蛋白酶原含量进行了检测。用肠激酶激活这些酶原，并用BAPNA（N-α-苯甲酰-DL-精氨酸-4-硝基苯胺盐酸盐，Fluka AG公司）和SUPHEPA（琥珀酰-L-苯丙氨酸对硝基苯胺，Becton公司旗下Schwarz/Mann分部）作为底物来估计活性蛋白酶的量。糜蛋白酶原的激活比胰蛋白酶原的激活更快；在3小时内发生最大激活。在类似情况下，胰蛋白酶原的激活需要17小时。在炎症过程中，胰蛋白酶原和糜蛋白酶原的含量均显著下降。8小时内，胰蛋白酶原含量从正常静息大鼠胰腺的酶原含量下降了28.4%，糜蛋白酶原含量下降了44.9%。这表明在胰腺炎过程中酶原和/或活性蛋白酶被释放出来。在正常胰腺中，未发现胰蛋白酶原和糜蛋白酶原含量之间存在相关性，但在胰腺炎期间，酶原含量明显相关。炎症期间的这种相关性可能反映了存活胰腺组织的数量和合成速率。
