Lan R, Lumb B, Ryan D, Reeves P R
Department of Microbiology, The University of Sydney, Sydney, New South Wales 2006, Australia.
Infect Immun. 2001 Oct;69(10):6303-9. doi: 10.1128/IAI.69.10.6303-6309.2001.
Three genes, ipgD, mxiC, and mxiA, all in the invasion region of the Shigella virulence plasmid, were sequenced from strains representing a range of Shigella serotypes and from two enteroinvasive Escherichia coli (EIEC) isolates. The plasmids can be classified into two relatively homogeneous sequence forms which are quite distinct. pINV A plasmids are found in Shigella flexneri strains F6 and F6A, S. boydii strains B1, B4, B9, B10, B14, and B15, S. dysenteriae strains D3, D4, D6, D8, D9, D10, and D13, and the two EIEC strains (M519 and M520). pINV B plasmids are present in S. flexneri strains F1A, F2A, F3A, F3C, F4A, and FY, two S. boydii strains (B11 and B12), and S. sonnei. The D1 pINV plasmid is a recombinant with ipgD gene more closely related to those of pINV A but with mxiA and mxiC genes more closely related to those of pINV B. The phylogenetic relationships of the plasmid and those of the chromosomal genes of Shigella strains are largely consistent. The cluster 1 and cluster 3 strains tested (G.M. Pupo, R. Lan, and P. R. Reeves, Proc. Natl. Acad. Sci. USA 97:10567-10572, 2000) have pINV A and pINV B plasmids, respectively. However, of the three cluster 2 strains (B9, B11, and B15), B9 and B15 have pINV A while B11 has a pINV B plasmid. Those Shigella (D8 and D10 and S. sonnei) and EIEC strains which do not group with the main body of Shigella strains based on chromosomal genes were found to have plasmids belonging to one or the other of the two types and must have acquired these by lateral transfer.
从代表一系列志贺氏菌血清型的菌株以及两株侵袭性大肠杆菌（EIEC）分离株中，对志贺氏菌毒力质粒侵袭区域内的三个基因ipgD、mxiC和mxiA进行了测序。这些质粒可分为两种相对同质但截然不同的序列形式。pINV A质粒存在于福氏志贺氏菌菌株F6和F6A、鲍氏志贺氏菌菌株B1、B4、B9、B10、B14和B15、痢疾志贺氏菌菌株D3、D4、D6、D8、D9、D10和D13以及两株EIEC菌株（M519和M520）中。pINV B质粒存在于福氏志贺氏菌菌株F1A、F2A、F3A、F3C、F4A和FY、两株鲍氏志贺氏菌菌株（B11和B12）以及宋内志贺氏菌中。D1 pINV质粒是一种重组质粒，其ipgD基因与pINV A的更密切相关，但mxiA和mxiC基因与pINV B的更密切相关。质粒的系统发育关系与志贺氏菌菌株染色体基因的系统发育关系在很大程度上是一致的。所测试的第1组和第3组菌株（G.M. Pupo、R. Lan和P.R. Reeves，《美国国家科学院院刊》97:10567 - 10572，2000）分别具有pINV A和pINV B质粒。然而，在三株第2组菌株（B9、B11和B15）中，B9和B15具有pINV A，而B11具有pINV B质粒。那些基于染色体基因未与志贺氏菌菌株主体归为一类的志贺氏菌（D8和D10以及宋内志贺氏菌）和EIEC菌株，被发现具有属于这两种类型之一的质粒，并且必定是通过横向转移获得的。
