Whetten R, Sun Y H, Zhang Y, Sederoff R
Forest Biotechnology Group, North Carolina State University, Raleigh 27695, USA.
Plant Mol Biol. 2001 Sep;47(1-2):275-91.
Loblolly pine (Pinus taeda L.) is the most widely planted tree species in the USA and an important tree in commercial forestry world-wide. The large genome size and long generation time of this species present obstacles to both breeding and molecular genetic analysis. Gene discovery by partial DNA sequence determination of cDNA clones is an effective means of building a knowledge base for molecular investigations of mechanisms governing aspects of pine growth and development, including the commercially relevant properties of secondary cell walls in wood. Microarray experiments utilizing pine cDNA clones can be used to gain additional information about the potential roles of expressed genes in wood formation. Different methods have been used to analyze data from first-generation pine microarrays, with differing degrees of success. Disparities in predictions of differential gene expression between cDNA sequencing experiments and microarray experiments arise from differences in the nature of the respective analyses, but both approaches provide lists of candidate genes which should be further investigated for potential roles in cell wall formation in differentiating pine secondary xylem. Some of these genes seem to be specific to pine, while others also occur in model plants such as Arabidopsis, where they could be more efficiently investigated.
火炬松（Pinus taeda L.）是美国种植最广泛的树种，也是全球商业林业中的重要树种。该物种基因组规模大且世代时间长，给育种和分子遗传分析都带来了障碍。通过对cDNA克隆进行部分DNA序列测定来发现基因，是为研究调控松树生长发育机制（包括木材中与商业相关的次生细胞壁特性）建立分子研究知识库的有效手段。利用松树cDNA克隆进行的微阵列实验，可用于获取有关已表达基因在木材形成中潜在作用的更多信息。已采用不同方法分析第一代松树微阵列数据，取得的成功程度各异。cDNA测序实验和微阵列实验在差异基因表达预测上存在差异，这源于各自分析性质的不同，但两种方法都提供了候选基因列表，这些基因在分化中的松树次生木质部细胞壁形成中的潜在作用应进一步研究。其中一些基因似乎是松树特有的，而其他一些基因也存在于拟南芥等模式植物中，在这些模式植物中可以更有效地进行研究。
