Ruiz C J, Stone L M, McPheeters M, Ogura T, Böttger B, Lasher R S, Finger T E, Kinnamon S C
Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, CO 80523, USA.
Chem Senses. 2001 Sep;26(7):861-73. doi: 10.1093/chemse/26.7.861.
The differentiated taste bud is a complex end organ consisting of multiple cell types with various morphological, immunocytochemical and electrophysiological characteristics. Individual taste cells have a limited lifespan and are regularly replaced by a proliferative basal cell population. The specific factors contributing to the maintenance of a differentiated taste bud are largely unknown. Supporting isolated taste buds in culture would allow controlled investigation of factors relevant to taste bud survival. Here we describe the culture and maintenance of isolated rat taste buds at room temperature and at 37 degrees C. Differentiated taste buds can be sustained for up to 14 days at room temperature and for 3-4 days at 37 degrees C. Over these periods individual cells within the cultured buds maintain an elongated morphology. Further, the taste cells remain electrically excitable and retain various proteins indicative of a differentiated phenotype. Despite the apparent health of differentiated taste cells, cell division occurs for only a short period following plating, suggesting that proliferating cells in the taste bud are quickly affected by isolation and culture.
分化的味蕾是一种复杂的终末器官，由多种具有不同形态、免疫细胞化学和电生理特征的细胞类型组成。单个味觉细胞的寿命有限，会定期被增殖的基底细胞群体所取代。维持分化味蕾的具体因素在很大程度上尚不清楚。在培养中支持分离的味蕾将有助于对与味蕾存活相关的因素进行可控研究。在此，我们描述了在室温及37℃条件下分离的大鼠味蕾的培养和维持方法。分化的味蕾在室温下可维持长达14天，在37℃下可维持3 - 4天。在这些时间段内，培养的味蕾中的单个细胞保持伸长的形态。此外，味觉细胞仍具有电兴奋性，并保留各种表明分化表型的蛋白质。尽管分化的味觉细胞表面看起来健康，但细胞分裂仅在接种后短时间内发生，这表明味蕾中的增殖细胞很快受到分离和培养的影响。
