Jauregui H O, Bradford W D, Arstila A U, Kinney T D, Trump B F
Am J Pathol. 1975 Jul;80(1):33-52.
The morphologic characteristics of acute iron loading were studied in HeLa cells incubated in an iron-enriched Eagle's medium containing 500 mug/ml of iron. Chemical studies showed that ferritin synthesis was rapidly induced and the concentration of intracellular ferritin increased up to 72 hours. Closely coupled with an increase in HeLa cell ferritin was a marked decrease in the rate of cell multiplication. The significant ultrastructural findings of iron-induced HeLa cell injury are characterized by the appearance of both autophagic multivesicular and residual bodies over the first 72 hours of iron incubation. The prominence of multivesicular bodies was noted after only 4 hours' incubation, with iron and myelin figures first appearing after 6 hours. Thus, the partial arrest of cell multiplication was associated with an increase in cytoplasmic residual bodies containing iron and other debris. The distribution of intracellular ferritin within HeLa cells differs significantly from the distribution described previously in hepatic parenchymal cells. In HeLa cells, ferritin particles were confined to lysosomal vesicles and were not identified in cell sap, endoplasmic reticulum, or Golgi apparatus.
在富含铁的伊格尔培养基（含铁量为500微克/毫升）中培养的HeLa细胞中，对急性铁负荷的形态学特征进行了研究。化学研究表明，铁蛋白合成迅速被诱导，细胞内铁蛋白浓度在72小时内持续增加。与HeLa细胞铁蛋白增加密切相关的是细胞增殖速率显著下降。铁诱导的HeLa细胞损伤的重要超微结构发现的特征是，在铁孵育的最初72小时内出现自噬多泡体和残余小体。仅在孵育4小时后就注意到多泡体的突出，铁和髓鞘样结构在6小时后首次出现。因此，细胞增殖的部分停滞与含有铁和其他碎片的细胞质残余小体的增加有关。HeLa细胞内铁蛋白的分布与先前描述的肝实质细胞内的分布有显著差异。在HeLa细胞中，铁蛋白颗粒局限于溶酶体小泡，在细胞液、内质网或高尔基体中未发现。
