Yekeler H, Bitmiş K, Ozçelik N, Doymaz M Z, Calta M
Department of Pathology Medical School, Firat University, Elaziğ, Turkey.
Toxicol Pathol. 2001 Jul-Aug;29(4):492-7. doi: 10.1080/01926230152499980.
In this study, the possible toxic effects of Alternariol Methyl Ether (AME) and Tenuazonic Acid (TeA) produced by Alternaria species on esophagus of mice were investigated by using light and electron microscopy techniques. Mice were orally fed daily with AME or TeA for 10 months and at the end of this period, the effects of these chemicals on the esophageal mucosa were determined. By light microscopic examination, moderate and severe dysplasia characterized by loss of polarity, nuclear pleomorphism, and hyperchromasia were observed in TeA-treated animals. Electron-microscopic examination of TeA-treated mucosal epithelial cells revealed pyknosis in some nuclei, granulation and increase in chromatin mass, irregularities in the nuclear contours, vacuolization in nucleoplasms. and marked pleomorphism in the nuclei. In conclusion, our results suggested that TeA has higher toxicity as evidenced by dysplastic transformation.
在本研究中，运用光学显微镜和电子显微镜技术，研究了链格孢属物种产生的链格孢酚甲醚（AME）和细交链孢菌酮酸（TeA）对小鼠食管可能产生的毒性作用。小鼠每日经口投喂AME或TeA，持续10个月，在此阶段结束时，测定这些化学物质对食管黏膜的影响。通过光学显微镜检查，在TeA处理的动物中观察到中度和重度发育异常，其特征为极性丧失、核多形性和核深染。对TeA处理的黏膜上皮细胞进行电子显微镜检查发现，一些细胞核固缩、染色质颗粒化和质量增加、核轮廓不规则、核质空泡化以及细胞核明显多形性。总之，我们的结果表明，发育异常转变证明TeA具有更高的毒性。
