Sooriakumaran Prasanna, Henderson Alastair, Denham Philippa, Langley Stephen Em
Department of Urology, Royal Surrey County Hospital, Surrey, UK. sooriakumaran@ gmail.com
Int J Surg Pathol. 2009 Jun;17(3):238-43. doi: 10.1177/1066896908316902. Epub 2008 May 28.
Gene expression profiling by DNA microarray analysis is a technique with great promise in cancer biology. The multifocality and heterogeneity of many prostate cancers makes the collection of adequate biological samples for such profiling particularly challenging. Current methods, such as laser capture microdissection, are not widely available and can have significant limitations. In this article, a novel method of prostatic sampling, which does not affect the histopathological assessment of the surgical specimen and provides adequate RNA yield for microarray analysis is described. This method is simple, inexpensive, easily reproducible, and has been validated as having >95% sensitivity and 99% specificity for histological prediction of tissue obtained. This method can be adopted by other investigators to perform DNA microarray analysis on prostate tumors.
通过DNA微阵列分析进行基因表达谱分析是一种在癌症生物学领域极具前景的技术。许多前列腺癌的多灶性和异质性使得收集足够的生物样本进行此类分析颇具挑战性。目前的方法，如激光捕获显微切割，并不广泛可用且可能有显著局限性。本文描述了一种新型前列腺采样方法，该方法不影响手术标本的组织病理学评估，并能为微阵列分析提供足够的RNA产量。此方法简单、廉价、易于重复，并且已被验证对所获取组织的组织学预测具有>95%的敏感性和99%的特异性。其他研究人员可以采用此方法对前列腺肿瘤进行DNA微阵列分析。
