Tünnemann R, Mehlmann M, Süssmuth R D, Bühler B, Pelzer S, Wohlleben W, Fiedler H P, Wiesmüller K H, Gauglitz G, Jung G
Institute of Organic Chemistry, Institute of Physical Chemistry, University of Tübingen, Germany.
Anal Chem. 2001 Sep 1;73(17):4313-8. doi: 10.1021/ac010270f.
This paper describes the design, characterization, and use of an optical biosensor suited for the process control of biotechnological processes. The detector principle is based on reflectometric interference spectroscopy (RIfS). RIfS enables a label-free, product-specific monitoring, with a future outline for on-line process control. The potential of the RIfS biosensor is exemplified by the qualitative and quantitative monitoring of the microbial production of vancomycin-type glycopeptide antibiotics.
本文描述了一种适用于生物技术过程控制的光学生物传感器的设计、特性及应用。该探测器原理基于反射干涉光谱法（RIfS）。RIfS能够实现无标记、针对特定产物的监测，并为在线过程控制勾勒了未来蓝图。通过对万古霉素型糖肽类抗生素微生物生产的定性和定量监测，例证了RIfS生物传感器的潜力。
