Haddad N, Ostyn A, Karoui C, Masselot M, Thorel M F, Hughes S L, Inwald J, Hewinson R G, Durand B
Secteur des Mycobactéries, Unité des Zoonoses Bactériennes, Agence Française de Sécurité Sanitaire des Aliments, Maisons-Alfort, France.
J Clin Microbiol. 2001 Oct;39(10):3623-32. doi: 10.1128/JCM.39.10.3623-3632.2001.
The molecular fingerprints of 1,349 isolates of Mycobacterium bovis received between 1979 and August 2000 at Agence Française de Sécurité Sanitaire des Aliments (Afssa) have been obtained by spoligotyping. The majority of the isolates (1,266) were obtained from cattle living in France. An apparently high level of heterogeneity was observed between isolates. One hundred sixty-one spoligotypes were observed in total, of which 153 were from French isolates. The two predominant spoligotypes, designated BCG-like and GB54, accounted for 26 and 12% of the isolates, respectively. In addition, 84% of the spoligotypes were found fewer than 10 times. Analysis of the results by clustering and parsimony-based algorithms revealed that the majority of the spoligotypes were closely related. The predominant spoligotype was identical to that of the vaccine strain Mycobacterium bovis BCG, which was isolated in France at the end of the 19th century. Some spoligotypes were closely associated with restricted geographical areas. Interestingly, some spoligotypes, which were frequently observed in France, were also observed in neighboring countries. Conversely, few spoligotypes were common to France and England, and those that were shared were observed at very different frequencies. This last point illustrates the potential role for an international data bank, which could help trace the spread of M. bovis across national borders.
通过间隔寡核苷酸分型技术，已获得了1979年至2000年8月间法国食品安全局（Afssa）收到的1349株牛分枝杆菌分离株的分子指纹图谱。大多数分离株（1266株）来自生活在法国的牛。在分离株之间观察到明显较高的异质性。总共观察到161种间隔寡核苷酸分型，其中153种来自法国分离株。两种主要的间隔寡核苷酸分型，分别命名为卡介苗样和GB54，分别占分离株的26%和12%。此外，84%的间隔寡核苷酸分型出现次数少于10次。通过聚类和基于简约性的算法对结果进行分析表明，大多数间隔寡核苷酸分型密切相关。主要的间隔寡核苷酸分型与19世纪末在法国分离出的疫苗株牛分枝杆菌卡介苗的相同。一些间隔寡核苷酸分型与特定的地理区域密切相关。有趣的是，一些在法国经常观察到的间隔寡核苷酸分型在邻国也有发现。相反，法国和英国共有的间隔寡核苷酸分型很少，而且共享的那些在频率上差异很大。最后一点说明了国际数据库的潜在作用，它可以帮助追踪牛分枝杆菌在国界间的传播。
