Filliol I, Ferdinand S, Negroni L, Sola C, Rastogi N
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, F-97165 Pointe-à-Pitre Cedex, Guadeloupe.
J Clin Microbiol. 2000 Jul;38(7):2520-4. doi: 10.1128/JCM.38.7.2520-2524.2000.
Fingerprinting based on variable numbers of tandem DNA repeats (VNTR), a recently described methodology, was evaluated for molecular typing of Mycobacterium tuberculosis in an insular setting. In this study, VNTR fingerprinting was used alone or as a second-line test in association with spoligotyping, double-repetitive-element PCR (DRE-PCR), and IS6110 restriction fragment length polymorphism (RFLP) analysis, and the discriminatory power for each method or the combination of methods was compared by calculating the Hunter-Gaston discriminative index (HGI). The results obtained showed that in 6 out of 12 (50%) cases, VNTR-defined clusters were further subdivided by spoligotyping, compared to 7 out of 18 (39%) cases where spoligotyping-defined clusters were further subdivided by VNTR. When used alone, VNTR was the least discriminatory method (HGI = 0.863). Although VNTR was significantly more discriminatory when used in association with spoligotyping (HGI = 0.982), the combination of spoligotyping and DRE-PCR (HGI = 0.992) was still the most efficient among rapid, PCR-based methodologies, giving results comparable to IS6110 RFLP analysis. Nonetheless, VNTR typing may provide additional phylogenetical information that may be helpful to trace the molecular evolution of tubercle bacilli.
基于可变数目串联重复序列（VNTR）的指纹图谱技术是一种最近描述的方法，在一个岛屿环境中对结核分枝杆菌进行分子分型评估。在本研究中，VNTR指纹图谱技术单独使用，或作为二线检测方法与间隔寡核苷酸分型（spoligotyping）、双重复元件PCR（DRE-PCR）以及IS6110限制性片段长度多态性（RFLP）分析联合使用，通过计算Hunter-Gaston鉴别指数（HGI）比较每种方法或方法组合的鉴别能力。获得的结果显示，在12例（50%）病例中有6例，VNTR定义的簇被间隔寡核苷酸分型进一步细分，相比之下，在18例（39%）病例中有7例，间隔寡核苷酸分型定义的簇被VNTR进一步细分。单独使用时，VNTR是鉴别能力最低的方法（HGI = 0.863）。尽管VNTR与间隔寡核苷酸分型联合使用时鉴别能力显著更高（HGI = 0.982），但间隔寡核苷酸分型和DRE-PCR的组合（HGI = 0.992）在基于PCR的快速方法中仍然是最有效的，其结果与IS6110 RFLP分析相当。尽管如此，VNTR分型可能提供额外的系统发育信息，这可能有助于追踪结核杆菌的分子进化。
