Mircetić R N, Dodig S, Raos M, Petres B, Cepelak I
Faculty of Pharmacy and Biochemistry, Department of Medical Biochemistry and Haematology, Domagojeva 2, 10000, Zagreb, Croatia.
Clin Chim Acta. 2001 Oct;312(1-2):197-203. doi: 10.1016/s0009-8981(01)00622-2.
Magnesium (Mg) is involved in numerous physiological functions, including protein folding, intracellular signalling and enzyme catalysis. It acts as a smooth muscle relaxant. We decided to test changes of total Mg concentration in plasma, leukocytes and urine of 16 healthy children and 26 patients with intermittent asthma aged 3 to 14 years.
Samples were taken on the first day of broncho-obstruction and five days after the acute attack. During this period, patients were under salbutamol therapy.
Plasma Mg concentration in patients increased by about 40% (first day 0.58+/-0.05 mmol Mg/l and five days later 0.64+/-0.04 mmol Mg/l) compared with healthy children (0.42+/-0.04 mmol Mg/l). Leukocyte Mg concentrations showed significant changes. On the first day of broncho-obstruction, Mg in leukocytes significantly decreased by about 60% (1.16+/-0.31 mmol Mg/g protein) compared to healthy children (3.04+/-0.68 mmol Mg/g protein). Five days later, Mg values significantly increased (3.28+/-1.09 mmol Mg/g protein) and almost reached the values of the healthy group. Mg concentration in urine statistically decreased by about 30% (0.55+/-0.06 mmol Mg/mmol creatinine) on the first day of broncho-obstruction compared to healthy children (0.75+/-0.05 mmol Mg/mmol creatinine). Five days after the acute attack, Mg concentration in patients' urine (0.73+/-0.07 mmol Mg/mmol creatinine) was close to values of healthy children.
The results obtained indicate that the intracellular measurement of Mg concentration is relevant for estimation of magnesium concentration in the human organism. Hence, determination of Mg concentration in leukocytes may be used in evaluation of asthmatic pathology.
镁（Mg）参与众多生理功能，包括蛋白质折叠、细胞内信号传导和酶催化。它可作为平滑肌松弛剂。我们决定检测16名健康儿童和26名3至14岁间歇性哮喘患者血浆、白细胞和尿液中总镁浓度的变化。
在支气管阻塞的第一天和急性发作后五天采集样本。在此期间，患者接受沙丁胺醇治疗。
与健康儿童（0.42±0.04 mmol Mg/l）相比，患者的血浆镁浓度增加了约40%（第一天为0.58±0.05 mmol Mg/l,五天后为0.64±0.04 mmol Mg/l）。白细胞镁浓度出现显著变化。在支气管阻塞的第一天，白细胞中的镁与健康儿童（3.04±0.68 mmol Mg/g蛋白质）相比显著降低了约60%（1.16±0.31 mmol Mg/g蛋白质）。五天后，镁值显著升高（3.28±1.09 mmol Mg/g蛋白质），几乎达到健康组的值。与健康儿童（0.75±0.05 mmol Mg/mmol肌酐）相比，支气管阻塞第一天患者尿液中的镁浓度统计学上降低了约30%（0.55±0.06 mmol Mg/mmol肌酐）。急性发作五天后，患者尿液中的镁浓度（0.73±0.07 mmol Mg/mmol肌酐）接近健康儿童的值。
所得结果表明，细胞内镁浓度的测量对于评估人体中的镁浓度具有重要意义。因此，白细胞中镁浓度的测定可用于哮喘病理学的评估。
