枯草芽孢杆菌中转录和核糖体核糖核酸基因的组织

Organization of transfer and ribosomal ribonucleic acid genes in Bacillus subtilis.

作者信息

Moran C P, Bott K F

出版信息

J Bacteriol. 1979 Nov;140(2):742-4. doi: 10.1128/jb.140.2.742-744.1979.

DOI:10.1128/jb.140.2.742-744.1979

PMID:115849

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216706/

Abstract

The structural relationship between the transfer ribonucleic acid (tRNA) and the ribosomal RNA (rRNA) genes of Bacillus subtilis has been studied by restriction endonuclease analysis of total chromosomal deoxyribonucleic acid (DNA) and characterization of DNA fragments cloned in Escherichia coli. The DNA sequences encoding rRNA and tRNA were assayed by hybridization to radioactive RNA. The results support the conclusion that the tRNA genes are interspersed between and closely linked to the rRNA genes of B. subtilis. They probably do not appear between the 16S and 23S rRNA genes as in E. coli.

摘要

通过对枯草芽孢杆菌总染色体脱氧核糖核酸（DNA）进行限制性内切酶分析以及对克隆于大肠杆菌中的DNA片段进行表征，研究了枯草芽孢杆菌的转移核糖核酸（tRNA）与核糖体核糖核酸（rRNA）基因之间的结构关系。通过与放射性RNA杂交来测定编码rRNA和tRNA的DNA序列。结果支持了这样的结论：tRNA基因散布于枯草芽孢杆菌的rRNA基因之间并与之紧密相连。它们可能不像在大肠杆菌中那样出现在16S和23S rRNA基因之间。