Rudner R, Chevrestt A, Buchholz S R, Studamire B, White A M, Jarvis E D
Department of Biological Sciences, Hunter College, City University of New York, New York 10021.
J Bacteriol. 1993 Jan;175(2):503-9. doi: 10.1128/jb.175.2.503-509.1993.
Sequence analysis of cloned rescued DNA fragments from a Bacillus subtilis strain with an inserted recombinant plasmid in ribosomal operon rrnE revealed the presence of two tRNA genes for Met and Asp at the 3' end of the operon. Probing chromosomal DNA from a strain carrying a plasmid inserted in rrnD with a fragment containing the genetically unassigned cluster of 16 tRNA genes revealed that the cluster is located immediately following the rrnD operon. Our findings show that all 10 rrn operons in B. subtilis are associated with tRNA gene clusters.
对一株在核糖体操纵子rrnE中插入重组质粒的枯草芽孢杆菌菌株克隆拯救的DNA片段进行序列分析，结果显示在该操纵子的3'端存在两个分别编码甲硫氨酸和天冬氨酸的tRNA基因。用一个含有16个未定位tRNA基因的基因簇片段探测携带插入rrnD的质粒的菌株的染色体DNA，结果表明该基因簇紧接rrnD操纵子之后。我们的研究结果表明，枯草芽孢杆菌中的所有10个rrn操纵子都与tRNA基因簇相关。
