Bushway R J, Engdahl B S, Colvin B M, Hanks A R
J Assoc Off Anal Chem. 1975 Sep;58(5):965-70.
The rotenoids deguelin, B-dihydrorotenone, dehydrorotenone, rotenone, 6alpha beta, 12alpha beta-rotenolone, and tephrosin were chromatographed on 8-12 mum silica. A mobile phase of chloroform-isooctane (35+65) pumped at a flow rate of 1 ml/min through a 30 cm column was used and the absorbance of the eluate was monitored at 294 nm. Rotenone, B-dihydrorotenone, deguelin, and dehydrorotenone are completely resolved while 6alpha beta, 12alpha beta-rotenolone and tephrosin chromatograph as one peak. This method has potential as a preparative separation technique for rotenoids. Also described is a procedure to quantitatively measure rotenone in pesticide formulations. Samples were extracted with chloroform and chromatographed at a flow of 2.5 ml/min. The method is rapid (rotenone is eluted in 12 min) and reproducible.
鱼藤酮类化合物鱼藤素、β-二氢鱼藤酮、脱氢鱼藤酮、鱼藤酮、6αβ,12αβ-鱼藤醇酮和灰叶素在8 - 12μm硅胶上进行色谱分析。使用氯仿 - 异辛烷（35 + 65）的流动相，以1 ml/min的流速通过30 cm的色谱柱进行泵送，并在294 nm处监测洗脱液的吸光度。鱼藤酮、β-二氢鱼藤酮、鱼藤素和脱氢鱼藤酮能完全分离，而6αβ,12αβ-鱼藤醇酮和灰叶素在色谱图上表现为一个峰。该方法有潜力作为鱼藤酮类化合物的制备分离技术。还描述了一种定量测定农药制剂中鱼藤酮的方法。样品用氯仿萃取，并以2.5 ml/min的流速进行色谱分析。该方法快速（鱼藤酮在12分钟内洗脱）且具有重现性。
