Redman Zachary C, Brodnax Kaylan, Couture Jordan, Tomco Patrick L
Department of Chemistry, College of Arts and Sciences, University of Alaska Anchorage, 3211 Providence Dr., Anchorage, AK, 99508.
Chromatographia. 2021 Feb;84(2):207-214. doi: 10.1007/s10337-020-03987-9. Epub 2020 Nov 21.
The piscicide CFT Legumine is applied to freshwater systems around the world to control invasive fish species. Rotenone, a potent inhibitor of mitochondrial cellular respiration, is the active ingredient of the piscicide; however, other rotenoids of unknown persistence and toxicity account for an equivalent amount by weight. This work identified six distinct rotenoids in CFT Legumine using liquid chromatography coupled with high resolution orbitrap mass spectrometry and optimized a rapid surface water sampling procedure for their analysis. The rotenoids were identified as rotenone and its isomer deguelin, their 12α-hydroxylated products rotenolone and tephrosin, as well as 6α,12α-dehydrorotenone and 6α,12α-dehydrodeguelin. The optimized procedure, extraction with Spin-X nylon membrane microcentrifuge filters followed by elution with acetonitrile, achieved recoveries ranging from 101 - 107 % and 97 - 145 % for all six rotenoids at high (125 nM, ~50 ppb) and low (25 nM, ~10 ppb) concentrations of CFT Legumine, respectively. Overall, this method provides a rapid sampling procedure necessary for monitoring rotenoid persistence in surface water to ensure safe and efficacious application of the pesticide.
杀鱼剂CFT Legumine被应用于世界各地的淡水系统，以控制入侵鱼类物种。鱼藤酮是一种线粒体细胞呼吸的强效抑制剂，是该杀鱼剂的活性成分；然而，其他持久性和毒性未知的鱼藤酮类物质在重量上占同等比例。这项工作使用液相色谱与高分辨率轨道阱质谱联用技术，在CFT Legumine中鉴定出六种不同的鱼藤酮类物质，并优化了一种用于其分析的快速地表水采样程序。这些鱼藤酮类物质被鉴定为鱼藤酮及其异构体脱氢鱼藤酮、它们的12α-羟基化产物鱼藤醇酮和灰叶素，以及6α,12α-脱氢鱼藤酮和6α,12α-脱氢脱氢鱼藤酮。优化后的程序是先用Spin-X尼龙膜微量离心过滤器进行萃取，然后用乙腈洗脱，对于高浓度（125 nM，约50 ppb）和低浓度（25 nM，约10 ppb）的CFT Legumine，所有六种鱼藤酮类物质的回收率分别为101 - 107%和97 - 145%。总体而言，该方法提供了一种快速采样程序，这对于监测地表水鱼藤酮类物质的持久性以确保该农药的安全有效应用是必要的。
