由棒状噬菌体单个噬菌斑产生的白喉毒素抗原筛选试验的特性分析
Characterization of a screening test for diphtherial toxin antigen produced by individual plaques of corynebacteriophages.
作者信息
Welkos S L, Holmes R K
出版信息
J Clin Microbiol. 1979 Jun;9(6):693-8. doi: 10.1128/jcm.9.6.693-698.1979.
Abstract
A passive immune hemolysis assay has been developed to detect diphtherial toxin produced in individual plaques of tox+ corynebacteriophages. This assay permits rapid screening of large numbers of corynebacteriophages for their ability to code for diphtherial toxin or related antigens. The specificity of the assay and its potential usefulness for genetic studies of toxinogenesis have been demonstrated with well-characterized tox+ and tox- laboratory strains of corynebacteriophages.
摘要
已开发出一种被动免疫溶血试验，用于检测产毒的白喉棒状噬菌体单个噬菌斑中产生的白喉毒素。该试验可快速筛选大量白喉棒状噬菌体，以检测它们编码白喉毒素或相关抗原的能力。利用特性明确的产毒和不产毒白喉棒状噬菌体实验室菌株，已证明了该试验的特异性及其在毒素产生遗传学研究中的潜在用途。
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