[偏振光谱荧光法同时测定氨基酸注射液、动植物提取物中的酪氨酸和色氨酸]
[Simultaneous determination of tyrosine and tryptophan in amino acid injection, animal and plant extracts by polarization spectrofluorimetry].
作者信息
Du M, Tang B, Shen H X
机构信息
Department of Chemistry, Nankai University, Tianjin 300017.
出版信息
Yao Xue Xue Bao. 1997 Sep;32(9):695-8.
A new method for the simultaneous determination of tyrosine and tryptophan by polarization spectrofluorimetry has been developed. The interference of phenylalanine and light scattering of solvent were eliminated by using vertically crossed polarizers. The overlapping of fluorescence spectra of the two amino acids was solved with filters. The linear ranges of determination were 0.02-12.0 mg.L-1(tyrosine) and 0.01-2.50 mg.L-1(tryptophan). The mean recoveries of tyrosine and tryptophan in samples were 95.4% and 101.7% (in amino acid injection). 99.2% and 100.0% (in wild jujube kernel), 95.3% and 97.2% (in shrimp shell) respectively. The proposed method has been used to determine tyrosine and tryptophan in amino acid injection, extracts of wild jujube kernel and shrimp shells with satisfactory results.
建立了一种用偏振光谱荧光法同时测定酪氨酸和色氨酸的新方法。通过使用垂直交叉偏振器消除了苯丙氨酸的干扰和溶剂的光散射。用滤光片解决了两种氨基酸荧光光谱的重叠问题。测定的线性范围为0.02 - 12.0 mg·L⁻¹（酪氨酸）和0.01 - 2.50 mg·L⁻¹（色氨酸）。样品中酪氨酸和色氨酸的平均回收率在氨基酸注射液中分别为95.4%和101.7%，在酸枣仁中分别为99.2%和100.0%，在虾壳中分别为95.3%和97.2%。该方法已用于测定氨基酸注射液、酸枣仁提取物和虾壳中的酪氨酸和色氨酸，结果令人满意。
Zotero 插件