Xiao Jin, Ren Feng-lian, Song Ge, Liao Lü, Yu Wen-feng, Zeng Tao
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2007 Oct;27(10):2088-92.
A new method using alternating penalty trilinear decomposition algorithm coupled with excitation-emission matrix fluorometry has been developed for simultaneous resolution and determination of tyrosine, phenylalanine and tryptophan. Their correlation coefficients were 0.9987, 0.9995 and 0.9993 respectively. The contents of tyrosine, phenylalanine and tryptophan in Hibiscus syriacus L. leaves were also be determined by this method after being extracted by ultrasonic. The coefficients of variation and the recoveries of the three amino acids were 0.84%, 0.36%, 1.59% and 101.0%-92.7%, 106.5%-93.0%, 103.0%-95.0% respectively. All these show that this is a simple, fast and cridible method.
一种结合交替惩罚三线性分解算法和激发发射矩阵荧光法的新方法已被开发出来，用于同时分辨和测定酪氨酸、苯丙氨酸和色氨酸。它们的相关系数分别为0.9987、0.9995和0.9993。木槿叶中的酪氨酸、苯丙氨酸和色氨酸含量在经超声提取后，也用该方法进行了测定。三种氨基酸的变异系数和回收率分别为0.84%、0.36%、1.59%和101.0%-92.7%、106.5%-93.0%、103.0%-95.0%。所有这些都表明这是一种简单、快速且可靠的方法。
