[导数荧光偏最小二乘法同时测定注射液中色氨酸、酪氨酸和苯丙氨酸]
[Simultaneous determination of tryptophan, tyrosine and phenylalanine in injection by derivative fluorescent PLS method].
作者信息
Ding Y, Su Q, Wu Q
机构信息
Department of Chemistry, University of Science and Technology of China, 230026 Hefei.
出版信息
Guang Pu Xue Yu Guang Pu Fen Xi. 2001 Apr;21(2):212-4.
A new first-derivative fluorescent method by partial least squares (PLS) for the simultaneous determination of tryptophan, tyrosine and Phenylalanine was developed. The determination was carried out in KH2PO4-K2HPO4 buffer solution (pH = 7.4) and at the EX (excite) wavelength of 216.6 nm. The method has been applied to the determination of tryptophan, tyrosine and phenylalanine in compound amino acid injection, and the relative errors are within +/- 7.2%.
建立了一种基于偏最小二乘法（PLS）的新型一阶导数荧光法，用于同时测定色氨酸、酪氨酸和苯丙氨酸。测定在KH2PO4-K2HPO4缓冲溶液（pH = 7.4）中进行，激发波长为216.6 nm。该方法已应用于复方氨基酸注射液中色氨酸、酪氨酸和苯丙氨酸的测定，相对误差在±7.2%以内。
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