Lazdins I, Holmes I H
J Gen Virol. 1979 Jul;44(1):123-33. doi: 10.1099/0022-1317-44-1-123.
In Vero cells infected with Bunyamwera virus there is a rapid inhibition of cell RNA and protein synthesis to levels of 30 and 3% respectively of the control rate, both the rate of inhibition and the time lag before its initiation being multiplicity dependent. Using u.v.-irradiated virus, investigation of the mechanism of inhibition of host cell protein synthesis indicates that synthesis of new virus components is required for inhibition to occur. Quantitative comparison of the proteins synthesized in infected cells shows that at higher m.o.i. synthesis of virus, as well as cellular proteins, is inhibited. Bunyamwera virus-infected Vero cells synthesized three virus-specific proteins identified as the structural virion proteins. Nucleoprotein is synthesized predominantly early in infection while the major envelope glycoprotein and the minor glycoprotein are synthesized predominantly late in the infection cycle.
在感染布尼亚姆韦拉病毒的非洲绿猴肾细胞（Vero细胞）中，细胞RNA和蛋白质合成迅速受到抑制，分别降至对照速率的30%和3%，抑制速率及其开始前的时间间隔均与感染复数相关。使用紫外线照射的病毒，对宿主细胞蛋白质合成抑制机制的研究表明，抑制作用的发生需要新病毒成分的合成。对感染细胞中合成的蛋白质进行定量比较发现，在较高感染复数时，病毒以及细胞蛋白质的合成均受到抑制。感染布尼亚姆韦拉病毒的Vero细胞合成了三种病毒特异性蛋白质，被鉴定为病毒粒子的结构蛋白。核蛋白主要在感染早期合成，而主要包膜糖蛋白和次要糖蛋白主要在感染周期后期合成。
