Yang B, Cao L, Xu Y, Sun Z X, Gu Z P
Shanghai Institue of Materia Medica, Chinese Academy of Sciences, Shanghai 200031.
Yao Xue Xue Bao. 1997 Aug;32(8):573-7.
The direct effects of gossypol on the serum-free primary cultures of rat luteal cells and cytotrophoblasts were observed. The results indicate that: (1) Gossypol affected the viability of the cultured rat luteal cells, LD50: 1.6 (0.4-2.9) micrograms.ml-1. (2) The basal secretions of progesterone were significantly inhibited at low and high concentrations. The activities of 3 beta-HSD, adenyl cyclase and hCG-stimulated progesterone production were inhibited by higher concentrations of gossypol. (3) Gossypol damaged cultured human decidual cells and cytotrophoblasts, the LD50 were 3.5(0.4-6.6) micrograms.ml-1 and 4.1(0.6-7.6) micrograms.ml-1, respectively. These results suggest that the luteolytic effect is the main mechanism of action for the termination of early pregnancy by gossypol, while the direct damaging effects on decidual cells and cytotrophoblasts may also play a role in the termination of early pregnancy.
观察了棉酚对大鼠黄体细胞和细胞滋养层细胞无血清原代培养物的直接作用。结果表明：(1)棉酚影响培养的大鼠黄体细胞的活力，半数致死剂量(LD50)：1.6(0.4 - 2.9)微克·毫升-1。(2)低浓度和高浓度时孕酮的基础分泌均受到显著抑制。较高浓度的棉酚抑制3β-羟类固醇脱氢酶(3β-HSD)、腺苷酸环化酶的活性以及人绒毛膜促性腺激素(hCG)刺激的孕酮生成。(3)棉酚损害培养的人蜕膜细胞和细胞滋养层细胞，LD50分别为3.5(0.4 - 6.6)微克·毫升-1和4.1(0.6 - 7.6)微克·毫升-1。这些结果提示，黄体溶解作用是棉酚终止早孕的主要作用机制，而对蜕膜细胞和细胞滋养层细胞的直接损害作用在早孕终止中也可能起作用。
